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Genome-wide identification of chicken bursae of Fabricius miRNAs in response to very virulent infectious bursal disease virus

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Abstract

Infectious bursal disease virus (IBDV) can cause a highly contagious immunosuppressive disease in young chickens. MicroRNAs (miRNAs) are crucial regulators of gene expression and are involved in the pathogenesis of IBDV infection. To investigate the roles of miRNA in chicken bursae of Fabricius in response to very virulent IBDV (vvIBDV) infection, RNA sequencing was performed to compare the small RNA libraries from uninfected and vvIBDV-infected group which was infected for 3 days. A total of 77 differentially expressed (DE) miRNAs were identified in BF, of which 42 DE miRNAs were upregulated and 35 DE miRNAs were downregulated. A gene ontology analysis showed that genes associated with cellular processes, cells, and binding were enriched. Moreover, pathway analyses suggested that apoptosis, T cell receptor signaling pathways, and chemokine signaling pathways may be activated following vvIBDV infection. In addition, we predicted the target genes of DE miRNAs and constructed an miRNA-mRNA regulatory network. In total, 189 pairs of miRNA-target genes were identified, comprising 67 DE miRNAs and 73 mRNAs. In this network, gga-miR-1684b-3p was identified with the highest fold change, as well as gga-miR-1788-3p and gga-miR-3530-5p showed a high degree of change. The above three miRNAs were considered to play vital roles in vvIBDV-host interactions. This study was the first to perform a comprehensive analysis of DE miRNAs in the bursa of Fabricius in response to vvIBDV infection, and it provided new insights into molecular mechanisms underlying vvIBDV infection and pathogenesis.

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Data availability

The raw data sets supporting the results of this article were available in the NCBI short reads archive and accession number is PRJNA635782. For information linking and citing, please refer to: https://www.ncbi.nlm.nih.gov/search/all/?term=PRJNA635782.

Abbreviations

vvIBDV:

Very virulent infectious bursal disease virus

BF:

Bursa of Fabricius

miRNA:

MicroRNA

TEM:

Transmission electron microscopy

DE:

Differentially expressed

PBS:

Phosphate-buffered saline

GO:

Gene ontology

KEGG:

Kyoto Encyclopedia of Genes and Genomes

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Acknowledgements

We would like to thank the Harbin Institute of Veterinary Medicine for supplying us the specific pathogen-free chickens and Novel Bioinformatics Ltd., Co for the support of bioinformatics analysis. We also thank GENE DENOVO for the support of uploading the sequencing data.

Funding

This work is supported by the National Science and Technology Support Program in Rural Areas of the12th Five-Year Plan [Grant 2015BAD12B01].

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Authors and Affiliations

  1. College of Veterinary Medicine, Northeast Agricultural University, Changjiang Road No. 600, Xiang Fang District, Harbin, People’s Republic of China

    Xuewei Huang, Yue Li, Xiaona Wang, Junyan Zhang, Li Wang, Han Zhou, Yanping Jiang, Wen Cui, Xinyuan Qiao, Yijing Li & Lijie Tang

  2. College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, People’s Republic of China

    Xuewei Huang

  3. Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, People’s Republic of China

    Xiaona Wang, Li Wang, Han Zhou, Yanping Jiang, Wen Cui, Xinyuan Qiao, Yijing Li & Lijie Tang

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  1. Xuewei Huang
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Correspondence to Lijie Tang.

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Ethical approval

Animal experiments were carried out in accordance with the recommendations in the institutional and national guidelines for animal were and use. The protocol was approved by the Committee on the Ethics of Animal Experiments of Northeast Agricultural University, Harbin, China (2016NEFU-315, 13 April 2017).

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Huang, X., Li, Y., Wang, X. et al. Genome-wide identification of chicken bursae of Fabricius miRNAs in response to very virulent infectious bursal disease virus. Arch Virol 167, 1855–1864 (2022). https://doi.org/10.1007/s00705-022-05496-6

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