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Molecular characterization of a new fusarivirus infecting Botryosphaeria dothidea, the causal agent of pear ring rot disease

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Abstract

Here, a novel mycovirus, tentatively designated as “Botryosphaeria dothidea fusarivirus 2” (BdFV2), was discovered in Botryosphaeria dothidea strain JZ-3. The complete genome sequence is 6,271 nucleotides (nt) in length, excluding the poly(A) tail, and contains two putative open reading frames (ORFs). The larger ORF1 encodes a polypeptide of 1,552 amino acids (aa) with conserved RNA-dependent RNA polymerase (RdRp) domains and a viral helicase domain. The ORF1-encoded polypeptide shares 19.47–78.70% sequence identity with those of other fusariviruses and shares the highest sequence identity (78.70%) with the corresponding protein aa sequences of Neofusicoccum luteum fusarivirus 1 (NlFV1) isolate CBS110299. The small ORF2 encodes a hypothetical protein with 479 aa, which is predicted to contain a chromosome segregation protein SMC domain of unknown function. Sequence alignments and phylogenetic analysis indicated that BdFV2 is a distinct member of the recently established family Fusariviridae. BdFV2 appears to be a novel fusarivirus infecting a pathogenic B. dothidea strain that causes pear ring rot disease.

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Funding

This research was supported by the National Natural Science Foundation of China (31972321, 31471862), the Earmarked Fund for Pear Modern Agro-industry Technology Research System (CARS-28-16).

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Authors and Affiliations

  1. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China

    Ying He, Qi Zou, Shanshan Li, Haodong Zhu, Ni Hong, Guoping Wang & Liping Wang

  2. Key Lab of Plant Pathology of Hubei Province, Wuhan, 430070, Hubei, China

    Ying He, Qi Zou, Shanshan Li, Haodong Zhu, Ni Hong, Guoping Wang & Liping Wang

Authors
  1. Ying He
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  2. Qi Zou
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  3. Shanshan Li
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  4. Haodong Zhu
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  5. Ni Hong
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  6. Guoping Wang
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  7. Liping Wang
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Correspondence to Liping Wang.

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Handling Editor: Ioly Kotta-Loizou.

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He, Y., Zou, Q., Li, S. et al. Molecular characterization of a new fusarivirus infecting Botryosphaeria dothidea, the causal agent of pear ring rot disease. Arch Virol 167, 1893–1897 (2022). https://doi.org/10.1007/s00705-022-05492-w

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  • DOI: https://doi.org/10.1007/s00705-022-05492-w

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