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Complete genome sequence of a novel fusarivirus from the phytopathogenic fungus Fusarium sp.

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Abstract

Fusarium diseases include wilts, blights, rots, and cankers of many horticultural, field, ornamental, and forest crops in both agricultural and natural ecosystems, and they significantly hinder food plant production. Here, we describe a novel mycovirus, tentatively designated as “Fusarium fusarivirus 1” (FuFV1), which was discovered in an isolate of the phytopathogenic fungus Fusarium sp. FuFV1 has a positive-sense single-stranded RNA (+ssRNA) genome of 6,391 nucleotides (nt) containing three open reading frames (ORFs). ORF1 encodes a large polypeptide of 1,501 amino acids (aa) with conserved RNA-dependent RNA polymerase (RdRp) and helicase (Hel) domains. ORF2, overlapping ORF1 by 122 nucleotides, encodes a polypeptide with a conserved Smc domain. The third and smaller ORF (ORF3) encodes a polypeptide with an unknown function. BLASTp analysis of the ORF1-encoded polypeptide revealed that FuFV1 shares the highest aa sequence similarity (68.5% identity, E-value 0.0) with Fusarium poae fusarivirus 1 (FpFV1, genus Alphafusarivirus). Phylogenetic analysis of the RdRp and helicase (Hel) sequences indicated that FuFV1 clustered closely with FpFV1 in a separate branch within the clade containing members of the genus Alphafusarivirus. Based on these results, we propose that FuFV1 should be considered a novel mycovirus belonging to the genus Alphafusarivirus of the family Fusariviridae.

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Data availability

The dataset generated during the current study is available in the GenBank database under the accession number OP729394.

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Funding

This study was funded by Yunnan Tobacco Company, Kunming Branch (KYN202202).

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Author notes

  1. Fuhong Gao and Rex Frimpong Anane contributed equally to this study.

Authors and Affiliations

  1. Yunnan Tobacco Company Kunming Branch, No. 523, Beijing Road, Panlong District, Kunming, 650051, Yunnan, China

    Fuhong Gao & Zhengling Liu

  2. Department of Biochemistry and Molecular Biology, Monash Biomedicine Discovery Institute, Monash University, 23 Innovation Walk, Clayton, VIC, 3800, Australia

    Rex Frimpong Anane

  3. Key Laboratory for Agricultural Biodiversity for Pest Management of China, Ministry of Education, Yunnan Agricultural University, No. 95, Jinhei Road, Panlong District, Kunming, 650201, Yunnan, China

    Shaomei Zi, Shangyun Li, Zefen Yang, Bifan Chu, Xingquan Chen & Mingfu Zhao

  4. College of Agronomy and Biotechnology, Yunnan Agricultural University, No. 95, Jinhei Road, Panlong District, Kunming, 650201, Yunnan, China

    Shangyun Li, Zefen Yang & Zeli Chen

  5. Quality Standard and Testing Technology Research Institute, Yunnan Academy of Agricultural Sciences, Beijing Road, Kunming, 2238650205, NoYunnan, China

    Zeli Chen

Authors
  1. Fuhong Gao
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  2. Rex Frimpong Anane
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  3. Zhengling Liu
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  4. Shaomei Zi
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  5. Shangyun Li
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  6. Zefen Yang
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  7. Bifan Chu
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  8. Xingquan Chen
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  9. Zeli Chen
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  10. Mingfu Zhao
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Corresponding authors

Correspondence to Zeli Chen or Mingfu Zhao.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Gao, F., Anane, R.F., Liu, Z. et al. Complete genome sequence of a novel fusarivirus from the phytopathogenic fungus Fusarium sp.. Arch Virol 168, 248 (2023). https://doi.org/10.1007/s00705-023-05872-w

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  • DOI: https://doi.org/10.1007/s00705-023-05872-w

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