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Molecular characterization of a novel fusarivirus infecting the plant-pathogenic fungus Botryosphaeria dothidea

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Abstract

A novel virus, Botryosphaeria dothidea fusarivirus 1 (BdFV1), was isolated from a fungal strain, SDAU11-86 of Botryosphaeria dothidea, and its complete genome sequence was determined. BdFV1 has a single-stranded positive-sense (+ssRNA) genome with 6,179 nucleotides, excluding the poly(A) tail. The genome of BdFV1 contains two putative open reading frames (ORFs). The first ORF encodes a large polyprotein of 1,544 amino acids (aa) with conserved RNA-dependent RNA polymerase and viral helicase domains. The second ORF encodes a putative 481-aa protein with unknown function. Sequence comparisons and phylogenetic analysis suggested that BdFV1 is a novel mycovirus belonging to the newly proposed family “Fusariviridae”. This is the first report of a +ssRNA mycovirus in B. dothidea.

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Acknowledgements

The authors are grateful to Dr. Huanting Liu (University of St Andrews, UK) for help in improving the manuscript.

Funding

This research was supported by the National Key R&D Program of China (2017YFD0600102-7 and 2017YFD0201103), the National Natural Science Foundation of China (31770684), and the Natural Science Foundation of Shandong Province (ZR2017MC042).

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Authors and Affiliations

  1. Shandong Research Center for Forestry Harmful Biological Control Engineering and Technology, College of Plant Protection, Shandong Agricultural University, Tai’an, 271018, Shandong, People’s Republic of China

    Wenyi Liu, Xiaojing Yu, Yingtong Zhao, Bangling He & Huixiang Liu

  2. State Key Laboratory of Agricultural Microbiology, The Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People’s Republic of China

    Wenyi Liu, Du Hai, Fan Mu, Jiatao Xie & Daohong Jiang

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  1. Wenyi Liu
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  2. Du Hai
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  3. Fan Mu
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  4. Xiaojing Yu
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  9. Huixiang Liu
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Correspondence to Huixiang Liu.

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Liu, W., Hai, D., Mu, F. et al. Molecular characterization of a novel fusarivirus infecting the plant-pathogenic fungus Botryosphaeria dothidea. Arch Virol 165, 1033–1037 (2020). https://doi.org/10.1007/s00705-020-04554-1

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  • DOI: https://doi.org/10.1007/s00705-020-04554-1

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