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Phylogenetic analysis of lumpy skin disease virus isolates in Russia in 2019-2021

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Abstract

Lumpy skin disease continues to pose a threat to countries in the East and Asia-Pacific regions. Although only occasional LSDV outbreaks have been reported recently in Russia, these have been mainly restricted to the Far East region of the country. An increase in the number of outbreaks in South East Asia has been attributed to recombinant vaccine-like LSDV strains. In this scenario, it is epidemiologically important to perform phylogenetic analysis to track the distribution of LSDV worldwide at the genetic level to understand routes of migration and molecular evolution patterns. In this study, we investigated the RPO30 and GPCR gene regions of LSDV isolates associated with outbreaks in 2019-2021 in Siberia and the Far East region of Russia. The inferred phylogeny confirms the recombinant origin of these sequenced isolates. Based on sequences of these selected loci, the isolates from 2019 differed from isolates detected in Russia in the past and from isolates from Asian countries, while the isolates from 2020 and 2021 exhibited a high degree of similarity to the Asian isolates. These findings indicate that recombinant LSDV strains continue to persist and additionally point to the establishment of a specific lineage of recombinant isolates in the region. Full genome sequencing is still needed to gain more information about how the circulating isolates are related to each other.

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Fig. 1
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Data availability

The datasets presented in this study are published in the GenBank database. The names of the isolates and accession number(s) can be found in the article in Table 1.

Abbreviations

LSDV:

Lumpy skin disease virus

PCR:

Polymerase chain reaction

ORF:

Open reading frame

kbp:

Kilobase pair

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Acknowledgements

The authors thank Shalina K, Prutnikov P and Zinyakov N for technical assistance. The authors appreciate A. Van Schalkwyk’s comments on the manuscript.

Funding

This work was supported by grant no. 075-15-2021-1054 from the Ministry of Education and Science of Russia to implement the objectives of the Federal Scientific and Technical Program for the Development of Genetic Technologies during 2019-2027.

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Authors and Affiliations

  1. Federal Center for Animal Health, Vladimir, Russia

    Alena Krotova, Ali Mazloum, Olga Byadovskaya & Alexander Sprygin

Authors
  1. Alena Krotova
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  2. Ali Mazloum
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  3. Olga Byadovskaya
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  4. Alexander Sprygin
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Contributions

A.S. and O.B. designed the study and revised the draft. A.K. and A.M. performed the experiments. A.K. and O.B. collected the samples. A.M. and A.S. drafted the manuscript. All authors revised and approved the paper for publication.

Corresponding author

Correspondence to Alexander Sprygin.

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Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Ethics statement

No animals were sacrificed for this study.

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Handling Editor: Tim Skern.

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Krotova, A., Mazloum, A., Byadovskaya, O. et al. Phylogenetic analysis of lumpy skin disease virus isolates in Russia in 2019-2021. Arch Virol 167, 1693–1699 (2022). https://doi.org/10.1007/s00705-022-05487-7

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  • DOI: https://doi.org/10.1007/s00705-022-05487-7

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