Abstract
In the present work, we report the discovery and complete genome sequence of a novel partitivirus identified from Brassica campestris L. ssp. chinensis, which we have named “Brassica campestris chinensis cryptic virus 1” (BCCV1). Next-generation sequencing (NGS) combined with adapter-ligation-mediated amplification allowed assembly of the full-length genome sequence of BCCV1. The genome of BCCV1 contains two dsRNA segments, dsRNA1 (1595 bp) and dsRNA2 (1591 bp), which encode a conserved RNA-dependent RNA polymerase (RdRp) and a putative capsid protein (CP), respectively. Homology searches and phylogenetic analysis of the 479-aa RdRp and 438-aa CP showed that BCCV1 is a new member of the genus Deltapartitivirus, family Partitiviridae. This is the first report of the identification of a member of the family Partitiviridae in Brassica campestris L. ssp. chinensis.
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Acknowledgements
This work was supported by the National Key Research and Development Program of China (2017YFD0101803) and Bulk Vegetable Industry Technology Systems of China (CARS-23-G-26).
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The complete genome sequence of BCCV1 has been deposited in the NCBI database with accession numbers MW026405 and MW026406.
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705_2021_5041_MOESM4_ESM.tif
Supplementary file4 Fig. S1 Confirmation of the presence of the BCCV1 genome using RT-PCR. The primer pair CVRdF/CVRdR was used for dsRNA1 (1293 bp), CVCPF/CVCPR for dsRNA2 (1012 bp), and BraActinF/BraActinR for the Brassica campestris L. ssp. chinensis actin gene (326 bp). Deionized water was used as a control (TIF 2096 KB)
705_2021_5041_MOESM5_ESM.tif
Supplementary file5 Fig. S2 Multiple alignment of the 5’ nucleotide sequences of BCCV1 and other putative partitivirus. SaCV1, Sinapis alba cryptic virus 1 (NC_030243 & NC_030245); CCV3, carnation cryptic virus 3 (NC_034513 & NC_034523); PCV-2, pepper cryptic virus 2 (NC_034159 & NC_034167); CiLCV, Citrullus lanatus cryptic virus (KY081285.1 & KY081284.1); BCCV1, Brassica campestris chinensis cryptic virus 1 (TIF 5639 KB)
705_2021_5041_MOESM6_ESM.tif
Supplementary file6 Fig. S3 Multiple alignment of the amino acid sequences of the RdRp (A) and CP (B) with putative partitiviruses. The conserved amino acids are highlighted in dark shading. BCCV1, Brassica campestris chinensis cryptic virus 1; CiLCV, Citrullus lanatus cryptic virus (APT68925.1); SaCV1, Sinapis alba cryptic virus 1 (YP_009255398.1); CCV3, carnation cryptic virus 3 (YP_009362091.1); PCV-2, pepper cryptic virus 2 (AVL84364.1); PeCV, persimmon cryptic virus (YP_006390091.1) (TIF 2692 KB)
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Tang, L., Song, L., Lin, C. et al. Complete nucleotide sequence of a novel partitivirus from Brassica campestris L. ssp. chinensis. Arch Virol 166, 1775–1778 (2021). https://doi.org/10.1007/s00705-021-05041-x
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DOI: https://doi.org/10.1007/s00705-021-05041-x