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Complete genome sequence of a novel partitivirus from the entomogenous fungus Beauveria bassiana in China

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Abstract

In this study, we report a novel double-stranded RNA (dsRNA) virus, Beauveria bassiana partitivirus 3 (BbPV-3), derived from the entomogenous fungus Beauveria bassiana isolate RCEF5853 from China. The genome of BbPV-3, whose sequence was determined by metagenomic sequencing, RT-PCR, and RACE cloning, comprises two dsRNA genome segments that are 1,856 and 1,719 bp long. The first segment contains a single ORF (ORF-1) encoding a 584-amino-acid-long protein (66.05 kDa) with a conserved RNA-dependent RNA polymerase (RdRp) motif. The second segment also has a single ORF (ORF-2) encoding a 500-amino-acid-long coat protein (CP) (55.9 kDa). The CP and RdRp sequences showed highest identity of 43.4% and 60.2%, respectively, to those of Colletotrichum eremochloae partitivirus 1. Phylogenetic analysis of the RdRp domain of the polyprotein revealed that BbPV-3 grouped together with the members of the genus Epsilonpartitivirus. Hence, we proposed that Beauveria bassiana partitivirus 3 is a novel member of the proposed genus Epsilonpartitivirus.

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Funding

This work was supported by the National Natural Science Foundation of China (grant nos. 31772226, 31471821 and 30770008).

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Author notes

  1. Najie Shi and Guogen Yang contributed equally to this study and share first authorship.

Authors and Affiliations

  1. Anhui Provincial Key Laboratory of Microbial Pest Control, Anhui Agricultural University, Hefei, 230036, China

    Najie Shi, Ping Wang, Yulong Wang, Deshui Yu & Bo Huang

  2. School of Plant Protection, Anhui Agricultural University, Hefei, 230036, China

    Guogen Yang

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  1. Najie Shi
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  2. Guogen Yang
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  3. Ping Wang
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  4. Yulong Wang
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  5. Deshui Yu
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  6. Bo Huang
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Correspondence to Bo Huang.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Handling Editor: Robert H.A. Coutts.

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Shi, N., Yang, G., Wang, P. et al. Complete genome sequence of a novel partitivirus from the entomogenous fungus Beauveria bassiana in China. Arch Virol 164, 3141–3144 (2019). https://doi.org/10.1007/s00705-019-04428-1

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  • DOI: https://doi.org/10.1007/s00705-019-04428-1

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