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Development of an antigen-capture enzyme-linked immunosorbent assay for diagnosis of Aleutian mink disease virus

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Abstract

Aleutian mink disease (AMD), caused by Aleutian mink disease virus (AMDV), is a very important infectious disease of mink. Currently, elimination of antibody- or antigen-positive animals is the most successful strategy for eradicating AMD, but the claw-cutting method of blood sampling is difficult to perform and painful for the animal. In this study, we aimed to establish an antigen capture enzyme-linked immunosorbent assay (AC-ELISA) method for the efficient detection of AMDV antigens using fecal samples. A purified mouse monoclonal antibody (mAb) was used as the capture antibody, and a rabbit polyclonal antibody (pAb) was used as the detection antibody. The assay was optimized by adjusting a series of parameters. Using a cutoff value of 0.205, the limit of detection of the AC-ELISA for strain AMDV-G antigen was 2 μg/mL, and there was no cross-reaction with other mink viruses. The intra- and inter-assay standard deviations were below 0.046, and the correlation of variance (CV) values were 1.24–7.12% when testing fecal samples. Compared with conventional PCR results, the specificity and sensitivity were 91.5% and 90.6%, respectively, and the concordance rate between the two methods was 91.1%.

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Availability of data and material

The datasets generated during the current study are available from the corresponding author on reasonable request.

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Funding

This work was supported by the National Natural Science Foundation of China (No. 31700140).

Author information

Authors and Affiliations

  1. Institute for Laboratory Animal Research, Guizhou University of Traditional Chinese Medicine, Guiyang, 550025, China

    Taofeng Lu, Yanjun Wu & Shuguang Wu

  2. State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 678 Haping Road, Harbin, 150069, China

    Yuanzhi Wang, Lili Zhao & Hongyan Chen

Authors
  1. Taofeng Lu
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  2. Yuanzhi Wang
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  3. Yanjun Wu
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  4. Lili Zhao
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  5. Shuguang Wu
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  6. Hongyan Chen
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Contributions

Conceptualization, Taofeng lu and Yuanzhi Wang; funding acquisition, Hongyan Chen and Taofeng Lu; investigation, Yuanzhi Wang; methodology, Taofeng Lu; project administration, Lili Zhao; supervision, Lili Zhao and Hongyan Chen; visualization, Taofeng Lu and Yanjun Wu; writing – original draft, Taofeng Lu; writing – review and editing, Taofeng Lu and Shuguang Wu.

Corresponding author

Correspondence to Hongyan Chen.

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Conflict of interest

The authors declared no potential conflicts of interest.

Ethical approval

The animal experiment was approved by Harbin Veterinary Research Institute (2017-009).

Informed consent

Informed consent was obtained from individual farmers for the samples used in this study.

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Handling Editor: Ana Cristina Bratanich.

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Lu, T., Wang, Y., Wu, Y. et al. Development of an antigen-capture enzyme-linked immunosorbent assay for diagnosis of Aleutian mink disease virus. Arch Virol 166, 83–90 (2021). https://doi.org/10.1007/s00705-020-04850-w

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  • DOI: https://doi.org/10.1007/s00705-020-04850-w

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