Abstract
Psittacine beak and feather disease (PBFD) is characterised by degenerative feather, feather dystrophy, and beak deformity. Sometimes acute forms can lead to fatal cases in nestlings. The worldwide distribution of this disease affects numerous species of parrots with an average prevalence of 40%, including in Taiwan. The pathogen of PBFD is beak and feather disease virus (BFDV), which is a single-stranded circular DNA virus, circovirus. To date, hemagglutination and PCR assays have been routinely used to detect this virus. In this study, both the replication-associated protein (Rep) and the structural capsid protein (Cap) were expressed and then used as antigens for the production of monoclonal antibodies. Conserved epitopes recognised by the anti-Cap and anti-Rep monoclonal antibodies were determined to be NFEDYRI and LSALKKM, respectively. Clinical samples collected from different species of parrots were tested by hemagglutination, PCR, and anti-Cap antigen-capture ELISA assays and the positive rates were the same at 49%. Thus, this anti-Cap antigen-capture ELISA is able to be used for the rapid identification of BFDV-infected birds in a non-invasive manner.
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Acknowledgements
This study was supported by grants from the Ministry of Science and Technology, Taiwan (MOST 105-2313-B-005-030 and MOST 103-2313-B-005-039) and RD106015 from Chang Bing Show Chwan Memorial Hospital. We thank Dr. Kirby for the editing of this manuscript.
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All authors declare no conflict of interest. All procedures performed in this study involving animals were approved under a permit issued by the Animal Ethics Committee of the National Chung Hsing University, Taiwan.
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Handling Editor: Sheela Ramamoorthy.
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Ho, CF., Huang, SW., Chan, KW. et al. Development of an antigen-capture ELISA for beak and feather disease virus. Arch Virol 163, 145–151 (2018). https://doi.org/10.1007/s00705-017-3596-6
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DOI: https://doi.org/10.1007/s00705-017-3596-6