Abstract
Aeromonas hydrophila is an important finfish pathogen, besides being an opportunistic human pathogen. In the present study, the genomes of three A. hydrophila-specific phages, CF8, PS1, and PS2, were isolated, characterized and sequenced. Transmission electron microscopy showed that all three phages had typical Myoviridae morphology. The linear dsDNA genomes of CF8, PS1, and PS2 were 238,150 bp, 237,367 bp, and 240,447 bp in length, with a GC content of 42.2%, 38.8%, and 38.8%, respectively. The low sequence similarity (67.6% - 69.8% identity with 27.0% - 29.0% query coverage) to other phage genomes in the NCBI database indicated the novel nature of the CF8, PS1, and PS2 genomes. A total of 244, 247, and 250 open reading frames (ORFs) were predicted in the CF8, PS1, and PS2 genome, respectively. During the annotation process, functional predictions were made for 28-31 ORFs, while the rest were classified as “hypothetical proteins” with yet unknown functions. Genes for tRNAs were also detected in all phage genomes. As all three phages in the present study had a very narrow host range with lytic activity against only one strain of A. hydrophila, these phages could be good candidates for phage typing applications. Moreover, the endolysin- and lytic-transglycosylase-encoding genes could be used for recombinant cloning and expression of anti-microbial proteins.
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19 May 2020
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Acknowledgements
The authors are grateful to Dr. Ashoo Toor, Assistant Professor, Punjab Agricultural University, Ludhiana, and Dr. Prabjeet Singh, Assistant Professor, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, for English and scientific language corrections.
Funding
This work was supported by RKVY Grant RKVY-11:I3 “Development of biotechnological intervention strategies to enhance the safety and shelf life of fishery products”.
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Rai, S., Tyagi, A., Kalia, A. et al. Characterization and genome sequencing of three Aeromonas hydrophila-specific phages, CF8, PS1, and PS2. Arch Virol 165, 1675–1678 (2020). https://doi.org/10.1007/s00705-020-04644-0
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DOI: https://doi.org/10.1007/s00705-020-04644-0