Abstract
The complete genome of the novel phage vB_EcoS_PHB17, which infects Shiga-toxin-producing Escherichia coli, was sequenced, revealing a linear double-stranded DNA genome of 48,939 bp with 46% GC content and protruding 150-bp 5ʹ cohesive termini. The genome contained 85 open reading frames, 28 of which were annotated with known functions. No tRNA-encoding genes were detected. Phylogenetic analysis suggested that phage PHB17 is a novel phage of family Siphoviridae.
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Funding
This work was supported by grants from the National Program on Key Research Project of China (2018YFD0500204), the Technology Base and Talents Special Program of Guangxi Province (2018AD09007), and the Innovation and Entrepreneurship Training Program for College Students in Hubei Province (no. S201910504011).
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Y. Chen drafted the manuscript. Y. Chen, S. Wang, X. Zhang, and X. Li performed the data analysis. P. Qian and H. Chen were responsible for experimental design. All authors reviewed and agreed to the publication of this manuscript.
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Chen, Y., Wang, S., Wang, Y. et al. Complete genome sequence of the novel phage vB_EcoS_PHB17, which infects Shiga-toxin-producing Escherichia coli. Arch Virol 164, 3111–3113 (2019). https://doi.org/10.1007/s00705-019-04402-x
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DOI: https://doi.org/10.1007/s00705-019-04402-x