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Molecular characterization of a new vitivirus discovered in a blueberry plant with green mosaic symptoms

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Abstract

A new virus belonging to the genus Vitivirus in the family Betaflexiviridae was identified by next-generation sequencing of a blueberry plant showing green mosaic symptoms. The genome organization of the virus, which is tentatively named “blueberry green mosaic-associated virus” (BGMaV), is typical of vitiviruses, with five open reading frames (ORFs) and a polyadenylated 3’ terminus. The ORFs code for the viral replicase, a 16K protein of unknown function, a movement protein, a coat protein (CP), and a nucleic acid binding protein. Phylogenetic analyses based on the deduced amino acid sequence of the CP and conserved motifs of the RNA-dependent RNA polymerase confirmed the taxonomic placement of BGMaV in the genus Vitivirus.

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Acknowledgements

The authors thank Dr. James J. Polashock for collecting the blueberry green mosaic symptomatic tissue and providing the photo in Figure 1A. Critical review and suggestions of Dr. James J. Polashock and Dr. Leslie L. Domier for improving the quality of the manuscript are greatly acknowledged.

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Author notes

  1. Thanuja Thekke-Veetil

    Present address: Department of Crop Sciences, University of Illinois Urbana-Champaign, Urbana, IL, 61801, USA

  2. Thien Ho

    Present address: Driscoll’s Inc., Watsonville, CA, 95076, USA

Authors and Affiliations

  1. Division of Agriculture, Department of Plant Pathology, University of Arkansas System, Fayetteville, AR, 72701, USA

    Thanuja Thekke-Veetil & Thien Ho

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  1. Thanuja Thekke-Veetil
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  2. Thien Ho
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Correspondence to Thanuja Thekke-Veetil.

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Handling Editor: Jesús Navas-Castillo.

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Thekke-Veetil, T., Ho, T. Molecular characterization of a new vitivirus discovered in a blueberry plant with green mosaic symptoms. Arch Virol 164, 2609–2611 (2019). https://doi.org/10.1007/s00705-019-04344-4

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  • DOI: https://doi.org/10.1007/s00705-019-04344-4

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