Abstract
We determined the complete genome sequence of a new virus infecting Ecballium elaterium (‘cohombrillo amargo’) plants, a weed species common on the borders of cultivated fields in the Mediterranean region. The genome of this virus is composed of two molecules of monocistronic positive-sense RNA, 6,934 and 3,501 nucleotides in length, excluding their poly(A) tails. The highest amino acid sequence similarity (50 % identity) in the Pro-Pol core region encoded by RNA 1 was observed in the corresponding protein of strawberry latent ringspot virus. Based on pairwise comparisons and phylogenetic analysis, this virus, tentatively named "cohombrillo-associated virus" (CoAV), appears to be a member of a new species in the genus Stralarivirus (family Secoviridae), for which the name “Stralarivirus elaterii” is proposed. This new virus has different putative cleavage patterns from members of other species belonging to this genus.
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Data availability
Author state that the sequences corresponding to RNA1 and RNA2 of CoAV have been submitted to the GenBank database under the accession numbers: OP019481 and OP019482, respectively. They will be available online as soon as possible. The sequences are also available in Supplemental file 1.
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Acknowledgements
We would like to express our deepest thanks to Hélène Sanfaçon (Summerland Research and Development Centre, Agriculture and Agri-Food Canada, British Columbia, Canada) for the stimulating discussion, for helping us to predict the amino acid at the substrate binding pocket site of the protease, and for her constant feedback on the prediction of the polyprotein cleavage sites. We want also to thank Mario Fon (mariogfon@gmail.com) for editing the final version of manuscript.
Funding
This article was funded by the European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie Grant Agreement No., 813542T.
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Conceptualization: AM, MAA, and LD. Experiments and analysis: AM. Writing: AM. Writing—review and editing: AM, MAA, and LD. Funding acquisition: YH.
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Maachi, A., Hernando, Y., Aranda, M.A. et al. Cohombrillo-associated virus: a novel virus infecting Ecballium elaterium plants. Arch Virol 168, 16 (2023). https://doi.org/10.1007/s00705-022-05669-3
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DOI: https://doi.org/10.1007/s00705-022-05669-3