Abstract
Disease caused by bovine leukemia virus (BLV) results in significant economic losses to the livestock industry. To date, there is only one report describing the strains found in Italy. BLV strains (n = 24), collected between 2012 and 2016 from four different Italian regions, were genetically analyzed by direct sequencing of a portion of the BLV env gene, and the sequences were compared with those in the GenBank database. The Italian BLVs clustered into genotypes G2, G4, G6, G7, and G8, revealing a high level of BLV genetic heterogeneity in Italy. This study provides a basis for further investigations into the evolutionary relationship between BLV strains.
References
King AMQ, Adams J, Carstens EB, Lefkowitz EJ (2012) Virus taxonomy: classification and nomenclature of viruses: Ninth report of the ICTV. Elsevier/Academic Press, Amsterdam, p 1344
Goff SP (2007) Retroviridae: the retroviruses and their replication. In: Knipe DM, Howley PM (eds) Fields virology. Lippincott Williams and Wilkins, Philadelphia, pp 1999–2069
Kettmann R, Burny A, Callebaut I, Droogmans L, Mammerickx M, Willems L, Portetelle D (1994) Bovineleukemia virus. In: Levy JA (ed) The Retroviridae, vol 3. Plenum Press, New York, pp 39–81
Willems L, Burny A, Collete D, Dangoisse O, Dequiedt F, Gatot JS, Kerkhofs P, Lefebvre L, Merezak C et al (2000) Genetic determinants of bovine leukemia virus pathogenesis. AIDS Res Hum Retroviruses 16:1787–1795
Martinez Cuesta L, Lendez PA, Nieto Farias MV, Dolcini GL, Ceriani MC (2018) Can bovine leukemia virus be related to human breast cancer? A review of the Evidence. J Mammary Gland Biol Neoplasia 23(3):101–107. https://doi.org/10.1007/s10911-018-9397-z
Lawson JS, Salmons B, Glenn WK (2018) Oncogenic viruses and breast cancer: mouse mammary tumor virus (MMTV), bovine leukemia virus (BLV), human papilloma virus (HPV), and epstein-barr virus (EBV). Front Oncol 8:1. https://doi.org/10.3389/fonc.2018.00001
Buehring GC, Shen H, Schwartz DA, Lawson JS (2017) Bovine leukemia virus linked to breast cancer in Australian women and identified before breast cancer development. PLoS One. https://doi.org/10.1371/journal.pone.0179367
Rola-Łuszczak M, Pluta A, Olech M, Donnik I, Petropavlovskiy M, Gerilovych A, Vinogradova I, Choudhury B, Kuźmak J (2013) The molecular characterization of bovine leukaemia virus isolates from Eastern Europe and Siberia and its impact on phylogeny. PLoS One. https://doi.org/10.1371/journal.pone.0058705
Aida Y, Murakami H, Takahashi M, Takeshima SN (2013) Mechanisms of pathogenesis induced by bovine leukemia virus as a model for human T-cell leukemia virus. Front Microbiol. 4:328. https://doi.org/10.3389/fmicb.2013.00328
Kabeya H, Ohashi K, Onuma M (2001) Host immune responses in the course of bovine leukemia virus infection. J Vet Med Sci. 63(7):703–708 (Review)
Rodriguez SM, Golemba MD, Campos RH, Trono K, Jones LR (2009) Bovine leukemia virus can be classified into seven genotypes: evidence for the existence of two novel clades. J Gen Virol 90(Pt 11):2788–2797. https://doi.org/10.1099/vir.0.011791-0
Pluta A, Rola-Łuszczak M, Kubiś P, Balov S, Moskalik R, Choudhury B (2017) Kuźmak molecular characterization of bovine leukemia virus from Moldovan dairy cattle. J Arch Virol 162(6):1563–1576
Sagata N, Yasunaga T, Tsuzuku-Kawamura J, Ohishi K, Ogawa Y, Ikawa Y (1985) Complete nucleotide sequence of the genome of bovine leukemia virus: its evolutionary relationship to other retroviruses. Proc Natl Acad Sci USA 82(3):677–681
Pluta A, Albritton LM, Rola-Łuszczak M, Kuźmak J (2018) Computational analysis of envelope glycoproteins from diverse geographical isolates of bovine leukemia virus identifies highly conserved peptide motifs. Retrovirology 15(1):2. https://doi.org/10.1186/s12977-017-0383-0 (PubMed PMID: 29310678; PubMed Central PMCID: PMC5759284)
Gillet N, Florins A, Boxus M, Burteau C, Nigro A, Vandermeers F et al (2007) Mechanisms of leukemogenesis induced by bovine leukemia virus: prospects for novel anti-retroviral therapies in humans. Retrovirology 4:18–50
Polat M, Takeshima SN, Aida Y (2017) Epidemiology and genetic diversity of bovine leukemia virus. Virol J 14(1):209
Camargos MF, Stancek D, Rocha MA, Lessa LM, Reis JK et al (2002) Partial sequencing of env gene of bovine leukaemia virus from Brazilian samples and phylogenetic analysis. J Vet Med B Infect Dis Vet Public Health 49:325–331
Camargos MF, Pereda A, Stancek D, Rocha MA, dos Reis JK et al (2007) Molecular characterization of the env gene from Brazilian field isolates of bovine leukemia virus. Virus Genes 34:343–350
Beier D, Blankenstain P, Marquard O, Kuzmak J (2001) Identification of different BLV provirus isolates by PCR RFLPA and DNA sequencing. Ber Munch Tierarztl Wsch 114:252–256
Mamoun RZ, Morisson M, Rebeyrotte N, Busetta B, Couez D et al (1990) Sequence variability of bovine leukemia virus env gene and its relevance to the structure and antigenicity of the glycoproteins. J Virol 64:4180–4188
Licursi M, Inoshima Y, Wu D, Yokoyama T, Gonzalez ET (2002) Genetic heterogeneity among bovine leukemia virus genotypes and its relation to humoral responses in hosts. Virus Res 86:101–110
Fechner H, Blankenstein P, Looman AC, Elwert J, Geue L et al (1997) Provirus variants of the bovine leukemia virus and their relation to the serological status of naturally infected cattle. Virology 237:261–269
Polat M, Takeshima SN, Hosomichi K, Kim J, Miyasaka T, Yamada K, Arainga M, Murakami T, Matsumoto Y, Barra Diaz V, Panei CJ, González ET, Kanemaki M, Onuma M, Giovambattista G, Aida Y (2016) A new genotype of bovine leukemia virus in South America identified by NGS-based whole genome sequencing and molecular evolutionary genetic analysis. Retrovirology 13(1):4
Lee E, Kim EJ, Joung HK, Kim BH, Song JY, Cho IS, Lee KK, Shin YK (2015) Sequencing and phylogenetic analysis of the gp51 gene from Korean bovine leukemia virus isolates. Virol J 12:64. https://doi.org/10.1186/s12985-015-0286-4
Polat M, Ohno A, Takeshima SN, Kim J, Kikuya M, Matsumoto Y, Mingala CN, Onuma M, Aida Y (2015) Detection and molecular characterization of bovine leukemia virus in Philippine cattle. Arch Virol 160(1):285–296. https://doi.org/10.1007/s00705-014-2280-3
Gautam S, Mishra N, Kalaiyarasu S, Jhade SK, Sood R (2018) Molecular characterization of bovine leukaemia virus (BLV) strains reveals existence of genotype 6 in cattle in India with evidence of a new subgenotype. Transbound Emerg Dis. https://doi.org/10.1111/tbed.12979
Mansky LM, Temin HM (1994) Lower mutation rate of bovine leukemia virus relative to that of spleen necrosis virus. J Virol 68:494–499
Willems L, Thienpont E, Kerkhofs P, Burny A, Mammerickx M et al (1993) Bovine leukemia virus an animal model for the study of intrastrain variability. J Virol 67:1086–1089
Coulston J, Naif H, Brandon R, Kumar S, Khan S et al (1990) Molecular cloning and sequencing of an Australian isolate of proviral bovine leukaemia virus DNA: comparison with other isolates. J Gen Virol 71:1737–1746
Molteni E, Agresti A, Meneveri R, Marozzi A, Malcovati M, Bonizzi L, Poli G, Ginelli E (1996) Molecular characterization of a variant of proviral bovine leukaemia virus (BLV). Zentralbl Veterinarmed B 43(4):201–211
Moratorio G, Obal G, Dubra A, Correa A, Bianchi S, Buschiazzo A, Cristina J, Pritsch O (2010) Phylogenetic analysis of bovine leukemia viruses isolated in South America reveals diversification in seven distinct genotypes. Arch Virol 155(4):481–489
Feliziani F, Lomolino R, Iscaro C, Costarelli S, Maresca C (2018) Italy finally complies with the requirements to be declared as free from Enzootic Bovine Leukosis. Large Anim Rev 24(3–9):3
Balasini D (1995) Zootecnica speciale. Edagricole, Bologna
Acknowledgements
We would like to thank Editage (http://www.editage.com) for English language editing.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare no conflict of interest.
Additional information
Handling Editor: William G Dundon.
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Bazzucchi, M., Iscaro, C., Casciari, C. et al. Molecular characterization of Italian bovine leukemia virus isolates reveals the presence of distinct phylogenetic clusters. Arch Virol 164, 1697–1703 (2019). https://doi.org/10.1007/s00705-019-04255-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00705-019-04255-4