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Genetic characterization of the complete genome of a mutant canine parvovirus isolated in China

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Abstract

A field canine parvovirus (CPV) strain, CPV-SH14, was previously isolated from an outbreak of severe gastroenteritis in Shanghai in 2014. The complete genome of CPV-SH14 was determined by using PCR with modified primers. When compared to other CPV-2 strains, several insertions, deletions, and point mutations were identified in the 5’ and 3’ UTR, with key amino acid (aa) mutations (K19R, E572K in NS1 and F267Y, Y324I and T440A in VP2) also being observed in the coding regions of CPV-SH14. These results indicated that significant and unique genetic variations have occurred at key sites or residues in the genome of CPV-SH14, suggesting the presence of a novel genetic variant of new CPV-2a. Phylogenetic analysis of the VP2 gene revealed that CPV-SH14 may have the potential to spread worldwide. In conclusion, CPV-SH14 may be a novel genetic variant of new CPV-2a, potentially with a selective advantage over other strains.

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Acknowledgements

This study was funded by the National Key Research and Development Program of China (No. 2016YFD0501003), the Fundamental Research Funds for the Central Institutes Program (No. 2016JB12) and the National Natural Science Foundation of China (No. 31502068).

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Authors and Affiliations

  1. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China

    Chuanfeng Li, Jingyu Tang, Zongyan Chen, Qi Li, Quan Wang, Chunchun Meng & Guangqing Liu

  2. College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, China

    Jingyu Tang & Yong Wang

  3. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, 730070, China

    Qi Li & Zhenhua Huang

Authors
  1. Chuanfeng Li
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  2. Jingyu Tang
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  3. Zongyan Chen
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  4. Qi Li
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  5. Zhenhua Huang
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  6. Quan Wang
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  7. Chunchun Meng
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  8. Yong Wang
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  9. Guangqing Liu
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Correspondence to Guangqing Liu.

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The authors declare that they have no competing interests.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Handling Editor: Sheela Ramamoorthy.

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Li, C., Tang, J., Chen, Z. et al. Genetic characterization of the complete genome of a mutant canine parvovirus isolated in China. Arch Virol 163, 521–525 (2018). https://doi.org/10.1007/s00705-017-3586-8

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  • DOI: https://doi.org/10.1007/s00705-017-3586-8

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