Abstract
Bisegmented dsRNA viruses that infect most or all isolates of apicomplexan parasite Cryptosporidium parvum are currently assigned to a single species, Cryptosporidium parvum virus 1, in genus Cryspovirus, family Partitiviridae. An analysis of existing sequence data suggested that the complete sequences of both cryspovirus genome segments, dsRNA1 and dsRNA2, had yet to be determined. We therefore set out to accomplish this for the virus strain that infects C. parvum isolate Iowa. The results suggest that several previous cryspovirus sequences are indeed truncated at one or both segment termini and also identify sequences at or near the termini that are conserved in both segments. Complete sequences of other cryspovirus strains, including ones from other Cryptosporidium species, are needed for refining their classification into one or more virus species.
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M. V. was supported in part by NIH Grant T32 GM007598 to the doctoral program in Molecules, Cells and Organisms at Harvard University. J.G.L. was supported in part by NIH Grants T32 AI07077 to the Sackler School of Graduate Biomedical Sciences Program in Immunology and T32 GM008448 to the School of Medicine Medical Scientist Training Program at Tufts University. M.L.N. was supported in part by a subcontract from NIH Grant R01 GM033050.
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705_2017_3385_MOESM1_ESM.tif
Fig. S1 Multiple sequence alignments of complete or putatively complete cryspovirus genome segments. Only sequences from the terminal regions of dsRNA1 (A) and dsRNA2 (B) are shown. The extents of sequences shown were chosen to encompass the putative start codon (shaded) in the 5′-terminal region or the stop codon (shaded) in the 3′-terminal region of each segment. The upstream AUG uniquely in frame in dsRNA2 of the C. felis 353 cryspovirus is also shaded. Sequences conserved between dsRNA1 and dsRNA2, as discussed in the main text, are overlined. *, wholly conserved residue at each alignment position (TIFF 631 kb)
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Vong, M., Ludington, J.G., Ward, H.D. et al. Complete cryspovirus genome sequences from Cryptosporidium parvum isolate Iowa. Arch Virol 162, 2875–2879 (2017). https://doi.org/10.1007/s00705-017-3385-2
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DOI: https://doi.org/10.1007/s00705-017-3385-2