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Complete genome sequence of a novel chrysovirus infecting Talaromyces neofusisporus

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Abstract

A double-stranded RNA (dsRNA) mycovirus was isolated from Talaromyces neofusisporus isolate HJ1-6 and named "Talaromyces neofusisporus chrysovirus 1" (TnCV1). It was found to consist of four dsRNA segments (TnCV1-1, TnCV1-2, TnCV1-3, and TnCV1-4) with lengths of 3595 bp, 3063 bp, 3054 bp, and 2876 bp, respectively. Sequence analysis showed that TnCV1-1 contains an open reading frame (ORF) encoding a putative RNA-dependent RNA polymerase (RdRp) of 1136 amino acids (aa), TnCV1-2 contains an ORF encoding a hypothetical protein of 906 aa, TnCV1-3 contains an ORF encoding a putative capsid protein (CP) of 938 aa, and TnCV1-4 contains an ORF encoding a hypothetical protein of 849 aa. The 5’ and 3’ untranslated regions (UTRs) of TnCV1-1, TnCV1-2, TnCV1-3, and TnCV1-4 showed a high degree of sequence similarity to each other. Phylogenetic analysis based on RdRp sequences suggested that TnCV1 is a new member of the genus Alphachrysovirus in the family Chrysoviridae. This is the first chrysovirus isolated from T. neofusisporus.

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Funding

This study was funded by the National Natural Science Foundation of China (32000017 and 31660012), the Guizhou Provincial Natural Science Foundation [ZK(2021)zhongdian030], the Department of Education of Guizhou Province (CN) [KY(2021)313], the Regional Common Diseases and Adult Stem Cell Transformation Research and Innovation Platform of Guizhou Provincial Department of Science and Technology [(2019)4008], and Guizhou Provincial Natural Science Foundation [ZK[2022] Key Program 039].

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Authors and Affiliations

  1. Key Laboratory of Endemic and Ethnic Diseases, Guizhou Medical University, Ministry of Education, 550004, Guiyang, China

    Yinhui Jiang, Xun Tian, Xiang Liu, Bi Yang, Qinrong Wang, Wenfeng Yu & Xiaolan Qi

  2. Key Laboratory of Medical Molecular Biology, Guizhou Medical University, 550004, Guiyang, Guizhou Province, China

    Yinhui Jiang, Xun Tian, Xiang Liu, Bi Yang, Qinrong Wang, Wenfeng Yu & Xiaolan Qi

  3. Department of Biology, School of Basic Medical Sciences, Guizhou Medical University, 550004, Guiyang, Guizhou Province, China

    Nianxue Wang

  4. Key Laboratory of Infectious Immune and Antibody Engineering of Guizhou Province, School of Biology and Engineering, Guizhou Medical University, 550004, Guiyang, Guizhou Province, China

    Jian Peng

  5. School of Environmental Sciences, University of Guelph, N1G 2W1, Guelph, ON, Canada

    Tom Hsiang

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  1. Yinhui Jiang
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Correspondence to Yinhui Jiang.

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Yinhui Jiang and Xun Tian contributed equally to this work.

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Jiang, Y., Tian, X., Liu, X. et al. Complete genome sequence of a novel chrysovirus infecting Talaromyces neofusisporus. Arch Virol 167, 2789–2793 (2022). https://doi.org/10.1007/s00705-022-05582-9

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  • DOI: https://doi.org/10.1007/s00705-022-05582-9

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