Complete genomic characterization of milk vetch dwarf virus isolates from cowpea and broad bean in Anhui province, China
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Cowpea and broad bean plants showing severe stunting and leaf rolling symptoms were observed in Hefei city, Anhui province, China, in 2014. Symptomatic plants from both species were shown to be infected with milk vetch dwarf virus (MDV) by PCR. The complete genomes of MDV isolates from cowpea and broad bean were sequenced. Each of them had eight genomic DNAs that differed between the two isolates by 10.7% in their overall nucleotide sequences. In addition, the MDV genomes from cowpea and broad bean were associated with two and three alphasatellite DNAs, respectively. This is the first report of MDV on cowpea in China and the first complete genome sequences of Chinese MDV isolates.
KeywordsComplete Genome Sequence Broad Bean Vigna Unguiculata Severe Stunting Reassortment Event
We thank Prof. M. J. Adams, Stevenage, Herts, UK, for correcting the English in the manuscript.
Compliance with ethical standards
This article does not contain any studies with human participants or animals performed by any of the authors and is in compliance with ethical standards for research.
Conflict of interest
The authors declare that they have no conflict of interest.
This study was funded by Special Fund for Agro-Scientific Research in the Public Interest (grant number: 201303028) and the International Science & Technology Cooperation Program of China (grant number: 2015DFA30700).
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