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Full-length M and L RNA sequences of tospovirus isolate 2009-GZT, which causes necrotic ringspot on tomato in China

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Abstract

The virus isolate 2009-GZT, collected from tomato in Guizhou province of China, was identified as a new member of the genus Tospovirus based on its S RNA sequence. Because its provisional name, “tomato necrotic spot virus” (TNSV), was identical to an already existing member of the genus Ilarvirus, 2009-GZT was renamed “tomato necrotic spot-associated virus” (TNSaV). In this study, the full-length sequences of the genomic M and L RNAs of TNSaV were determined and analyzed. The M RNA has 4,773 nucleotides (nt), encoding the NSm protein of 309 aa (34.4 kDa) in the viral (v) strand and the glycoprotein precursor (Gn/Gc) of 1123 aa (128 kDa) in the viral complementary (vc) strand. The NSm and Gn/Gc of TNSaV share the highest aa sequence identity (86.2 % and 86.9 %, respectively) with those of tomato zonate spot virus. The L RNA contains 8,908 nt and codes for the putative RNA-dependent RNA polymerase (RdRp) of 2885 aa (332 kDa) in the vc strand. The RdRp of TNSaV shares the highest aa sequence identity (85.2 %) with that of calla lily chlorotic spot virus (CCSV). Serological assays showed that TNSaV cross-reacts with rabbit antisera against watermelon silver mottle virus (WSMoV) NP and CCSV NP, indicating that TNSaV is a member of the WSMoV serogroup.

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    Authors and Affiliations

    1. Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, 9# Xueyun Rd, Wuhua prefecture, Kunming, 650223, Yunnan, People’s Republic of China

      Kuanyu Zheng, Yueyan Yin, Zhongkai Zhang & Jiahong Dong

    2. Yunnan Provincial Key Lab of Agricultural Biotechnology, 9# Xueyun Rd, Wuhua prefecture, Kunming, 650223, Yunnan, People’s Republic of China

      Kuanyu Zheng, Yueyan Yin, Zhongkai Zhang & Jiahong Dong

    3. Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, 9# Xueyun Rd, Wuhua prefecture, Kunming, 650223, Yunnan, People’s Republic of China

      Kuanyu Zheng, Yueyan Yin, Zhongkai Zhang & Jiahong Dong

    4. HongyunHonghe Group Limited Company, 181# Hongjin Road, Kunming, 650202, Yunnan, People’s Republic of China

      Hongguang Liu

    5. Department of Biotechnology, Asia University, Wufeng, Taichung, 41354, Taiwan

      Tsung-Chi Chen

    6. Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, 40402, Taiwan

      Tsung-Chi Chen

    7. Department of Plant Pathology, National Chung Hsing University, Taichung, 402, Taiwan

      Shyi-Dong Yeh

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    1. Kuanyu Zheng
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    2. Hongguang Liu
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    3. Yueyan Yin
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    5. Shyi-Dong Yeh
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    Correspondence to Jiahong Dong.

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    I have read and have abided by the statement of ethical standards for manuscripts submitted to Archives of Virology.

    Funding

    This study was partially supported by grants from the National Natural Science Foundation of China (31371919), Top Scientists Input Program of Yunnan (2013HA028), and Provincial Natural Science Foundation of Yunnan (2012CH007).

    Ethical approval

    This article does not contain any studies with human participants or animals performed by any of the authors.

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    The authors declare that they have no conflict of interest.

    Additional information

    K. Zheng and H. Liu contributed equally to this study.

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    Zheng, K., Liu, H., Yin, Y. et al. Full-length M and L RNA sequences of tospovirus isolate 2009-GZT, which causes necrotic ringspot on tomato in China. Arch Virol 161, 1411–1414 (2016). https://doi.org/10.1007/s00705-016-2788-9

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    • DOI: https://doi.org/10.1007/s00705-016-2788-9

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