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Genomic characterization of a novel torradovirus infecting Arctium lappa L. in China

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Abstract

Burdock (Arctium lappa L.) is not only a popular vegetable crop but also an important medicinal plant. In burdock plants with symptoms of leaf mosaic, a novel torradovirus tentatively named "burdock mosaic virus" (BdMV) was identified by high-throughput sequencing. The complete genomic sequence of BdMV was further determined using RT-PCR and the rapid amplification of cDNA ends (RACE) method. The genome is composed of two positive-sense single-stranded RNAs. RNA1 (6991 nt) encodes a polyprotein of 2186 aa, and RNA2 (4700 nt) encodes a protein of 201 aa and a polyprotein of 1212 aa that is predicted to be processed into one movement protein (MP) and three coat proteins (CPs). The Pro-Pol region of RNA1 and the CP region of RNA2 shared the highest amino acid sequence identity of 74.0% and 70.6%, respectively, with the corresponding sequences of lettuce necrotic leaf curl virus (LNLCV) isolate JG3. Phylogenetic analysis based on the amino acid sequences of the Pro-Pol and CP regions showed that BdMV clustered with other non-tomato-infecting torradoviruses. Taken together, these results suggest that BdMV is a new member of the genus Torradovirus.

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All data included in this study are available upon request by contact with the corresponding author.

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Funding

This work was supported by the National Natural Science Foundation of China, grant number 32160031, and partially supported by the Natural Science Foundation of Inner Mongolia, China, grant number 2019MS03021, the Natural Science Foundation of Heilongjiang Province, grant number YQ2020C005, and China Agriculture Research System of MOF and MARA.

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Authors and Affiliations

  1. College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, 010018, Hohhot, China

    Zhengnan Li & Lei Zhang

  2. College of Plant Protection, Northeast Agricultural University, 150030, Harbin, China

    Shuang Song

  3. Research Institute of Pomology, Chinese Academy of Agriculture Sciences, 125100, Liaoning, Xingcheng, China

    Xudong Fan

  4. College of Plant Protection, Gansu Agricultural University, 730070, Lanzhou, China

    Qiaolan Liang & Yahan Chen

  5. Inner Mongolia Key Laboratory of Characteristic Geoherbs Resources Protection and Utilization, College of Pharmacy, BaoTou Medical College, 014040, Baotou, China

    Minhui Li

Authors
  1. Zhengnan Li
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  2. Lei Zhang
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  3. Xudong Fan
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  4. Qiaolan Liang
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  5. Yahan Chen
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  6. Minhui Li
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  7. Shuang Song
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Correspondence to Shuang Song.

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This article does not contain any experiments involving humans or animals, and no ethical approval was required.

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Communicated by Ralf Georg Dietzgen

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Li, Z., Zhang, L., Fan, X. et al. Genomic characterization of a novel torradovirus infecting Arctium lappa L. in China. Arch Virol 168, 107 (2023). https://doi.org/10.1007/s00705-023-05736-3

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  • DOI: https://doi.org/10.1007/s00705-023-05736-3

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