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Detection and genetic variation analysis of grapevine fanleaf virus (GFLV) isolates in China

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Abstract

To investigate the prevalence and genetic variation of grapevine fanleaf virus (GFLV) in China, 142 grapevine samples from 13 provinces and regions were tested using DAS-ELISA, RT-PCR, and nested RT-PCR. Of the samples, 38 % tested positive for GFLV by DAS-ELISA, and 26.8 % tested positive by RT-PCR and nested RT-PCR. Movement protein (MP) and coat protein (CP) gene PCR products were cloned and sequenced. The MP or CP nucleotide and protein sequences shared identities that ranged from 94.9 % to 100 %. Phylogenetic analysis revealed that Chinese GFLV isolates obtained in this study were distinct from the isolates reported in GenBank.

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References

  1. Andret-Link P, Laporte C, Valat L (2004) Grapevine fanleaf virus: still a major threat to the grapevine industry. J Plant Pathol 86(3):183–195

    CAS  Google Scholar 

  2. Bashir NS, Hajizadeh M (2007) Survey for Grapevine fanleaf virus in vineyards of north-west Iran and genetic diversity of isolates. Australas Plant Pathol 36:46–52

    Article  Google Scholar 

  3. Bashir NS, Melcher U (2012) Population genetic analysis of Grapevine fanleaf virus. Arch Virol 157:1919–1929

    Article  Google Scholar 

  4. Bertolini E, García J, Yuste A, Olmos A (2010) High prevalence of viruses in table grape from Spain detected by real-time RT-PCR. Eur J Plant Pathol 128:283–287

    Article  Google Scholar 

  5. Boulila M (2007) Phylogeny and genetic recombination of Grapevine fanleaf virus isolates from naturally infected vineyards in Tunisia. Phytopathol Mediterr 46:285–294

    CAS  Google Scholar 

  6. Chen S, Zhou Y, Ye T, Hao L, Guo L, Fan Z, Li S, Zhou T (2014) Genetic variation analysis of Apple chlorotic leaf spot virus coat protein reveals a new phylogenetic type and two recombinants in China. Arch Virol 159(6):1431–1438

    Article  CAS  PubMed  Google Scholar 

  7. Chung CT, Niemela SA, Miller RH (1989) One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proc Natl Acad Sci USA 86:2172–2175

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  8. Fiore N, Zamorano A, Rivera L, Gonzalez F, Aballay E, Montealegre J, Pino AM (2011) Grapevine viruses in the Atacama region of Chile. J Phytopathol 159:743–750

    Article  CAS  Google Scholar 

  9. Gambino G, Bondaz J, Gribaudo I (2006) Detection and elimination of viruses in callus, somatic embryos and regenerated plantlets of grapevine. Eur J Plant Pathol 114:397–404

    Article  Google Scholar 

  10. Ge B, He Z, Zhang Z, Wang H, Li S (2014) Genetic variation in potato virus M isolates infecting pepino (Solanum muricatum) in China. Arch Virol 159(12):3197–3210

    Article  CAS  PubMed  Google Scholar 

  11. Hudson RR (2000) A new statistic for detecting genetic differentiation. Genetics 155:2011–2014

    PubMed Central  CAS  PubMed  Google Scholar 

  12. Librado P, Rozas J (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25:1451–1452

    Article  CAS  PubMed  Google Scholar 

  13. Liebenberg A, Freeborough MJ, Visser CJ, Bellstedt DU, Burger JT (2009) Genetic variability within the coat protein gene of Grapevine fanleaf virus isolates from South Africa and the evaluation of RT-PCR, DAS-ELISA and immunostrips as virus diagnostic assays. Virus Res 142:28–35

    Article  CAS  PubMed  Google Scholar 

  14. Martin DP, Lemey P, Lott M, Moulton V, Posada D, Lefeuvre P (2010) RDP3: a flexible and fast computer program for analysing recombination. Bioinformatics 26:2462–2463

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  15. MacKenzie DJ, McLean MA, Mukerji S, Green M (1997) Improved RNA extraction from woody plants for the detection of viral pathogens by reverse transcription-polymerase chain reaction. Plant Dis 81:222–226

    Article  CAS  Google Scholar 

  16. Mahfoudhi N, Harbi-Ben SM, Elair M, Selmi I, Ben Hamda H (2014) Prevalence of viruses infecting autochthonous grapevines in Tunisia. Tunisian J Plant Prot 9:111–118

    Google Scholar 

  17. Maliogka V, Dovas CI, Efthimiou K, Katis NI (2004) Detection and differentiation of Comoviridae species using a semi-nested RT-PCR and a phylogenetic analysis based on the polymerase protein. J Phytopathol 152:404–409

    Article  CAS  Google Scholar 

  18. Mekuria T, Martin RR, Naidu RA (2008) The occurrence of Grapevine fanleaf Virus in Washington State Vineyards. In: Proceedings of the 2nd annual national viticulture research conference, July 9–11, University of California, Davis

  19. Myles S, Boykob AR, Owens CL, Brown PJ, Grassi F, Aradhya MK, Prins B, Reynolds A, Jer-Ming Chia, Ware D, Carlos D, Bustamante, Buckler ES (2011) Genetic structure and domestication history of the grape. Proc Natl Acad Sci USA 108:3530–3535

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  20. Oliver JE, Vigne E, Fuchs M (2010) Genetic structure and molecular variability of Grapevine fanleaf virus populations. Virus Res 152:30–40

    Article  CAS  PubMed  Google Scholar 

  21. Poljua D, Sladonja B, Bubola M (2010) Incidence of viruses infecting grapevine varieties in Istria (Croatia). J Food Agric Environ 8:166–169

    Google Scholar 

  22. Pompe-Novak M, Gutierrez-Aguirre I, Vojvoda J, Blas M, Tomazic I, Vigne E, Fuchs M, Ravnikar M, Petrovic N (2007) Genetic variability within RNA2 of Grapevine fanleaf virus. Eur J Plant Pathol 117:307–312

    Article  CAS  Google Scholar 

  23. Reynard JS, Gugerli P (2012) Current status of major grapevine viruses in La Côte vineyards of Switzerland. In: 17th ICVG Congress, Davis (US), pp 7–14

  24. Rowhani A, Walker MA, Rokni S (1992) Sampling strategies for the detection of Grapevine fanleaf virus and the grapevine strain of Tomato ringspot virus. Vitis 31(1):35–44

    Google Scholar 

  25. Sanfacon H, Wellink J, Le Gall O, Karasev A, van der Vlugt R, Wetzel T (2009) Secoviridae: a proposed family of plant viruses within the order Picornavirales that combines the families Sequiviridae and Comoviridae, the unassigned genera Cheravirus and Sadwavirus, and the proposed genus Torradovirus. Arch Virol 154:899–907

    Article  CAS  PubMed  Google Scholar 

  26. Sanfacon H, Gorbalenya AR, Knowles NJ, Chen JP (2012) Order Picornavirales. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy. Classification and nomenclature of viruses. Ninth report of the international committee on taxonomy of viruses. Elsevier Inc., Amsterdam, pp 835–839

    Google Scholar 

  27. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30(12):2725–2729

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  28. Terlizzi F, Pisi A, Beber R, Fiore N, Zamorano A, Credi R, Ratti C (2015) Genetic variabitily of the movement and coat protein genes of Grapevine fanleaf virus isolates from Italy. J Gen Plant Pathol 81:63–67

    Article  CAS  Google Scholar 

  29. Wen WG, Cui JX, Sheng L (2007) Detection of Tobacco ringspot virus and Tomato ringspot virus by semi-nested RT-PCR. ACTA Phytophylacica Sinica 34(1):61–65

    CAS  Google Scholar 

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Acknowledgments

This work was supported by the earmarked fund of China Agriculture Research System (CARS-30-bc-3).

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Authors and Affiliations

  1. National Center for Eliminating Viruses from Deciduous Fruit Tree, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng, 125100, Liaoning, China

    Jun Zhou, Xudong Fan, Yafeng Dong, Zun ping Zhang, Fang Ren & Guojun Hu

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  1. Jun Zhou
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  2. Xudong Fan
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  3. Yafeng Dong
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  4. Zun ping Zhang
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  5. Fang Ren
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  6. Guojun Hu
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Correspondence to Yafeng Dong.

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Zhou, J., Fan, X., Dong, Y. et al. Detection and genetic variation analysis of grapevine fanleaf virus (GFLV) isolates in China. Arch Virol 160, 2661–2667 (2015). https://doi.org/10.1007/s00705-015-2566-0

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