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Identification of novel double-stranded RNA mycoviruses of Fusarium virguliforme and evidence of their effects on virulence

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Abstract

Virulence and double-stranded RNA (dsRNA) profiles of 44 isolates of Fusarium virguliforme were compared. When grouped according to dsRNA profiles, isolates with large dsRNAs were significantly (P≤0.05) less virulent than isolates without dsRNAs. High-throughput sequence analysis of total RNA prepared from cultures with large dsRNAs identified two novel RNA viruses with genome sequences of approximately 9.3 kbp, which were named Fusarium virguliforme dsRNA mycovirus 1 and Fusarium virguliforme dsRNA mycovirus 2. The new viruses were most closely related to a group of unclassified viruses that included viruses of F. graminearum and Phlebiopsis gigantea and are related to members of the family Totiviridae.

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References

  1. Al Rwahnih M, Daubert S, Urbez-Torres JR, Cordero F, Rowhani A (2011) Deep sequencing evidence from single grapevine plants reveals a virome dominated by mycoviruses. Arch Virol 156:397–403

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  2. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410

    CAS  PubMed  Google Scholar 

  3. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1996) Current protocols in molecular biology. Wiley, New York

    Google Scholar 

  4. Boland GJ (2004) Fungal viruses, hypovirulence, and biological control of Sclerotinia species. Can J Plant Pathol 26:6–18

    Article  Google Scholar 

  5. Brent KJ, Hollomon DW (2007) Fungicide resistance: the assessment of risk. FRAC Monograph No 2 second revised edition CropLife International, United Kingdom, pp 1–53

  6. Chiba S, Salaipeth L, Lin YH, Sasaki A, Kanematsu S, Suzuki N (2009) A novel bipartite double-stranded RNA mycovirus from the white root rot fungus Rosellinia necatrix: molecular and biological characterization, taxonomic considerations, and potential for biological control. J Virol 83:12801–12812

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  7. Chu YM, Jeon JJ, Yea SJ, Kim YH, Yun SH, Lee YW, Kim KH (2002) Double-stranded RNA mycovirus from Fusarium graminearum. Appl Environ Microbiol 68:2529–2534

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  8. Fehr WR, Caviness CE, Burmood DT, Pennington JS (1971) Stage of development descriptions for soybeans, Glycine max (L.) Merrill. Crop Sci 11:929–931

    Article  Google Scholar 

  9. Grabherr MG, Haas BJ, Yassour M, Levin JZ, Thompson DA, Amit I, Adiconis X, Fan L, Raychowdhury R, Zeng QD, Chen ZH, Mauceli E, Hacohen N, Gnirke A, Rhind N, di Palma F, Birren BW, Nusbaum C, Lindblad-Toh K, Friedman N, Regev A (2011) Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat Biotech 29:644–652

    Article  CAS  Google Scholar 

  10. Hartman GL, Huang YH, Nelson RL, Noel GR (1997) Germplasm evaluation of Glycine max for resistance to Fusarium solani, the causal organism of sudden death syndrome. Plant Dis 81:515–518

    Article  Google Scholar 

  11. Hartman GL, Hill CB (2010) Diseases of soybean and their management. In: Singh G (ed) Soybean: botany, Production and uses. CAB International, Wallingford, pp 276–299

    Chapter  Google Scholar 

  12. Horsfall JG, Barratt RW (1945) An improved grading system for measuring diseases. Phytopathology 35:655

    Google Scholar 

  13. Kozlakidis Z, Hacker CV, Bradley D, Jamal A, Phoon X, Webber J, Brasier CM, Buck KW, Coutts RHA (2009) Molecular characterisation of two novel double-stranded RNA elements from Phlebiopsis gigantea. Virus Genes 39:132–136

    Article  CAS  PubMed  Google Scholar 

  14. Kwon SJ, Lim WS, Park SH, Park MR, Kim KH (2007) Molecular characterization of a dsRNA mycovirus, Fusarium graminearum virus-DK21, which is phylogenetically related to hypoviruses but has a genome organization and gene expression strategy resembling those of plant potex-like viruses. Mol Cells 23:304–315

    CAS  PubMed  Google Scholar 

  15. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23:2947–2948

    Article  CAS  PubMed  Google Scholar 

  16. Lee KM, Yu J, Son M, Lee YW, Kim KH (2011) Transmission of Fusarium boothii mycovirus via protoplast fusion causes hypovirulence in other phytopathogenic fungi. PLoS ONE 6:e21629

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  17. Milgroom MG, Cortesi P (2004) Biological control of chestnut blight with hypovirulence: A critical analysis. Annu Rev Phytopath 42:311–338

    Article  CAS  Google Scholar 

  18. Nogawa M, Shimosaka M, Kageyama T, Okazaki M (1993) A double-stranded-RNA mycovirus from the plant-pathogenic fungus, Fusarium solani f. sp. robiniae. FEMS Microbiol Let 110:153–158

    Article  CAS  Google Scholar 

  19. Nogawa M, Kageyama T, Nakatani A, Taguchi G, Shimosaka M, Okazaki M (1996) Cloning and characterization of mycovirus double-stranded RNA from the plant pathogenic fungus, Fusarium solani f. sp. robiniae. Biosci Biotechnol Biochem 60:784–788

    Article  CAS  PubMed  Google Scholar 

  20. Nuss DL (1992) Biological control of chestnut blight: an example of virus mediated attenuation of fungal pathogenesis. Microbiol Rev 56:561–576

    CAS  PubMed Central  PubMed  Google Scholar 

  21. Roy KW, Rupe JC, Hershman DE, Abney TS (1997) Sudden death syndrome of soybean. Plant Dis 81:1100–1111

    Article  Google Scholar 

  22. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor

    Google Scholar 

  23. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Molec Biol Evol 24:1596–1599

    Article  CAS  PubMed  Google Scholar 

  24. Tzeng TH, Tu CL, Bruenn JA (1992) Ribosomal frameshifting requires a pseudoknot in the Saccharomyces cerevisiae double-stranded RNA virus. J Virol 66:999–1006

    CAS  PubMed Central  PubMed  Google Scholar 

  25. Wickner RB, Ghabrial SA, Nibert ML, Patterson JL, Wang CC (2012) Family Totiviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy: classification and nomenclature of viruses; Ninth Report of the International Committee on taxonomy of viruses. Elsevier Academic Press, Waltham, pp 639–650

    Google Scholar 

  26. Yu J, Kwon S-J, Lee K-M, Son M, Kim K-H (2009) Complete nucleotide sequence of double-stranded RNA viruses from Fusarium graminearum strain DK3. Arch Virol 154:1855–1858

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

We thank A. M. Dorrance, W. L. Pedersen, and X. B. Yang for providing F. virguliforme isolates, J. Chee-Sanford and K. N. Lambert for use of gel imaging systems, W. Chen, C. J. D’Arcy, and K. N. Paige for their research advice, and W. A. Maffit for assistance in total RNA purifications. Funding for this project was provided, in part, from the USDA NC-125 project, The Illinois Soybean Association, and from the University of Illinois at Urbana Champaign Campus Research Board.

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Authors and Affiliations

  1. Department of Crop Sciences, University of Illinois, Urbana, IL, USA

    Rita A. Marvelli, Houston A. Hobbs & Darin M. Eastburn

  2. Crop Genetics Research Unit, United States Department of Agriculture-Agricultural Research Service, Stoneville, MS, USA

    Shuxian Li

  3. United States Department of Agriculture-Agricultural Research Service, Department of Crop Sciences, University of Illinois, Urbana, IL, USA

    Nancy K. McCoppin, Leslie L. Domier & Glen L. Hartman

Authors
  1. Rita A. Marvelli
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  2. Houston A. Hobbs
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  3. Shuxian Li
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  4. Nancy K. McCoppin
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  5. Leslie L. Domier
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  6. Glen L. Hartman
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  7. Darin M. Eastburn
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Correspondence to Leslie L. Domier.

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Marvelli, R.A., Hobbs, H.A., Li, S. et al. Identification of novel double-stranded RNA mycoviruses of Fusarium virguliforme and evidence of their effects on virulence. Arch Virol 159, 349–352 (2014). https://doi.org/10.1007/s00705-013-1760-1

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  • DOI: https://doi.org/10.1007/s00705-013-1760-1

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