Abstract
The incomplete sequences of two large, 10–12 kbp, double-stranded RNAs (dsRNAs) found in the TW-2 isolate of the saprophytic fungus, Phlebiopsis gigantea (Pg) are reported. Both PgV-TW2 dsRNA1 and dsRNA2 potentially encode fusion proteins which are apparently expressed by a translational frameshifting mechanism. The C-terminal region of both predicted proteins was 21% identical and contained the eight motifs conserved in RNA-dependent RNA polymerases of dsRNA mycoviruses and had highest similarity with members of the family Totiviridae, but possibly do not form virions. The remainder of the N-terminal protein sequences predicted from the PgV-TW2 dsRNA1 and dsRNA2 sequences and the 3′-terminal nucleotide sequences of both dsRNAs had no homology with one another or any sequence in the database suggesting that individually both may be members of novel families of mycoviruses.
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Acknowledgements
Zisis Kozlakidis was supported by a Bursary from The Faculty of Natural Sciences, Imperial College and Caroline Hacker was supported by the Natural Environmental Research Council and the Forestry Commission. We also thank the Pakistan HEC for supporting the Ph.D studies of Atif Jamal.
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The nucleotide sequence data reported in this article has been assigned the accession numbers AM111O96 and AM111097 for PgV-TW2 dsRNA1 and PgV-TW2 dsRNA2 respectively.
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Kozlakidis, Z., Hacker, C.V., Bradley, D. et al. Molecular characterisation of two novel double-stranded RNA elements from Phlebiopsis gigantea . Virus Genes 39, 132–136 (2009). https://doi.org/10.1007/s11262-009-0364-z
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DOI: https://doi.org/10.1007/s11262-009-0364-z