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Identification and characterization of a type III Trichomonas vaginalis virus in the protozoan pathogen Trichomonas vaginalis

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Abstract

A type III Trichomonas vaginalis virus, which may be involved in transcriptional regulation of the major surface protein gene P270 of the protozoan pathogen Trichomonas vaginalis, was purified and characterized in the present study. The complete 4844-base-pair complementary DNA sequence of the viral genome reveals overlapping cap and pol genes with a putative ribosomal frame-shifting signal within the overlap region. The type III virus is related more closely to the type II virus than to the type I virus in the sequence of its ribosomal frameshift signal and in its capsid protein. Phylogenetic analysis revealed that these viruses could be grouped in the same clade as a genus distantly related to other genera in the family Totiviridae. Virus-induced P270 gene expression was only evident in Trichomonas vaginalis cells infected with either a type II or type III virus, but not with a type I virus. These findings suggest that transcription of the P270 gene is likely regulated by viral factors common to type II and type III viruses and thus provides important information for future investigation of virus-host interactions.

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Acknowledgements

This work was supported by National Science Council (Grant No. NSC97-2320-B-001-005-MY3), and Academia Sincia, Taiwan, Republic of China. Electron microscopy was performed with the help of Hsiu-Jung Ma of the EM core facility at the Institute of Biomedical Sciences. We thank Dr. J.F. Alderete (School of Molecular Biosciences, Washington State University) for providing monoclonal antibody C20A3. We also appreciate the help of Mr. Douglas Platt in reading and correcting the manuscript.

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Authors and Affiliations

  1. Division of Infectious Diseases, Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, ROC

    Irina N. Bessarab, Rui Nakajima, Hsing-Wei Liu & Jung-Hsiang Tai

Authors
  1. Irina N. Bessarab
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  2. Rui Nakajima
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  3. Hsing-Wei Liu
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  4. Jung-Hsiang Tai
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Correspondence to Jung-Hsiang Tai.

Additional information

The sequence data from this manuscript have been deposited with GenBank Data Libraries under accession no. AF325840.

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Bessarab, I.N., Nakajima, R., Liu, HW. et al. Identification and characterization of a type III Trichomonas vaginalis virus in the protozoan pathogen Trichomonas vaginalis . Arch Virol 156, 285–294 (2011). https://doi.org/10.1007/s00705-010-0858-y

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  • DOI: https://doi.org/10.1007/s00705-010-0858-y

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