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Identification of a perchloric acid-soluble protein (PSP)-like ribonuclease from Trichomonas vaginalis

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Abstract

A perchloric acid-soluble protein (PSP), named here tv-psp1, was identified in Trichomonas vaginalis. It is expressed under normal culture conditions according to expressed sequence tag (EST) analysis. On the other hand, Tv-PSP1 protein was identified by mass spectrometry with a 40% of identity to human PSP (p14.1). Polyclonal antibodies against recombinant Tv-PSP1 (rTv-PSP1) recognized a single band at 13.5 kDa in total protein parasite extract by SDS-PAGE and a high molecular weight band analyzed by native PAGE. Structural analysis of Tv-PSP1, using dynamic light scattering, size exclusion chromatography, and circular dichroism spectroscopy, showed a trimeric structure stable at 7 M urea with 38% α-helix and 14% β-sheet in solution and a molecular weight of 40.5 kD. Tv-PSP1 models were used to perform dynamic simulations over 100 ns suggesting a stable homotrimeric structure. Tv-PSP1 was located in the nucleus, cytoplasm, and hydrogenosomes of T. vaginalis, and the in silico analysis by Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) showed interactions with RNA binding proteins. The preliminary results of RNA degradation analysis with the recombinant Tv-PSP1 showed RNA partial deterioration suggesting a possible putative ribonuclease function.

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Acknowledgements

We acknowledge Biol. Anel Edith Lagunes Guillén, M.Sc. Laura Isabel Vazquez Carrillo, QFB Leticia Avila-González, and Brenda Herrera Villalobos for their technical assistance.

Funding

This work was supported by UACM and a grant from CONACyT (83808) Mexico (to M.E.A.S.). AVO was supported by a scholarship from ICyTDF (ICyTDF/SRI/70/2011).

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Authors and Affiliations

  1. Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México (UACM), San Lorenzo # 290, Col. Del Valle, 03100, Ciudad de Mexico, Mexico

    Alma Villalobos-Osnaya & María Elizbeth Alvarez-Sanchez

  2. Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Universidad # 3000, 04510, Ciudad de Mexico, Mexico

    Georgina Garza-Ramos

  3. Departamento de Química, Universidad Autónoma Metropolitana, Apartado Postal 55-534, Iztapalapa, 09340, Ciudad de Mexico, Mexico

    Iris N. Serratos

  4. Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, 62209, Cuernavaca, Morelos, Mexico

    César Millán-Pacheco

  5. Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Av. IPN # 2508, Col. San Pedro Zacatenco, Delg. Gustavo A. Madero, 07360, Ciudad de Mexico, Mexico

    Arturo González-Robles & Rossana Arroyo

  6. Universidad Mexiquense del Bicentenario, Unidad de Estudios Superiores Tultitlan, Av. Ex Hacienda de Portales s/n, Col. Villa Esmeralda, 54910, Tultitlan, Estado de México, Mexico

    Laura Itzel Quintas-Granados

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  1. Alma Villalobos-Osnaya
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  8. María Elizbeth Alvarez-Sanchez
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Villalobos-Osnaya, A., Garza-Ramos, G., Serratos, I.N. et al. Identification of a perchloric acid-soluble protein (PSP)-like ribonuclease from Trichomonas vaginalis. Parasitol Res 117, 3639–3652 (2018). https://doi.org/10.1007/s00436-018-6065-6

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