Abstract
In the hyperarid Atacama Desert, water availability plays a crucial role in allowing plant survival. Along with scant rainfall, marine advective fog frequently occurs along the coastal escarpment fueling isolated mono-specific patches of Tillandsia vegetation. In this study, we investigate the lipid biomarker composition of the bromeliad Tillandsia landbeckii (CAM plant) to assess structural adaptations at the molecular level as a response to extremely arid conditions. We analyzed long-chain n-alkanes and fatty acids in living specimens (n = 59) collected from the main Tillandsia dune ecosystems across a 350 km coastal transect. We found that the leaf wax composition was dominated by n-alkanes with concentrations (total average 160.8 ± 91.4 µg/g) up to three times higher than fatty acids (66.7 ± 40.7 µg/g), likely as an adaptation to the hyperarid environment. Significant differences were found in leaf wax distribution (Average Chain Length [ACL] and Carbon Preference Index [CPI]) in the northern zone relative to the central and southern zones. We found strong negative correlations between fatty acid CPI and n-alkane ACL with precipitation and surface evaporation pointing at fine-scale adaptations to low moisture availability along the coastal transect. Moreover, our data indicate that the predominance of n-alkanes is reflecting the function of the wax in preventing water loss from the leaves. The hyperarid conditions and good preservation potential of both n-alkanes and fatty acids make them ideal tracers to study late Holocene climate change in the Atacama Desert.
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Introduction
The Atacama Desert is considered to be one of the oldest and most arid deserts on Earth and is an extreme habitat for life development (McKay et al. 2003; Hartley et al. 2005). The aridity in the Atacama Desert is caused principally by cold coastal waters that characterize the Humboldt Current and the upwelling of cold deep water to the surface, which creates an atmospheric thermal inversion preventing precipitation along the coastal zone (Houston and Hartley 2003). The aridity is intensified by the rain shadow effect of the Andean Mountains that blocks the transfer of moisture from the Amazon basin (Houston and Hartley 2003; Cereceda et al. 2008; Garreaud et al. 2008). As a result, the Atacama Desert has a hyperarid core characterized by mean annual precipitation lower than 2 mm between 19 and 22°S (Ritter et al. 2019; Voigt et al. 2020). Although the extremely low moisture supply to the Atacama Desert is not constant and varies geographically (e.g., latitude, distance inland and elevation), there is a clear latitudinal trend of the scarce precipitation increasing toward the north along the hyperarid core and over the coastal area (Schulz et al. 2011; Jaeschke et al. 2019; Reyers et al. 2021).
Besides infrequent rainfalls, marine advective fog from the Pacific Ocean is the most important source of moisture along the Atacama Desert (Cereceda et al. 2008; Lobos Roco et al. 2018; Schween et al. 2020; Del Río et al. 2021). Frequent coastal fog events are generally considered to be restricted to distances of up to a few kilometers from the coast and elevations of about 100–1200 m (Caviedes 1973; Prohaska 1973; García et al. 2021; Del Río et al. 2021). Beyond these elevations, hyperarid conditions limit the growth and survival of plants on the landscape and extensive areas without any vegetation exist, especially in the hyperarid core of Atacama Desert (Arroyo et al. 1988; Rundel and Dillon 1998; Schulz et al. 2011; Knief et al. 2020; Merklinger et al. 2020). Orographic fog oases are common along this coast and allow the development of unique plant communities (called lomas) of surprisingly high biodiversity in northern Chile and southern Peru (Rundel et al. 1991; Pinto et al. 2006; Merklinger et al. 2020). Fog corridors are unequally distributed and the hills are often isolated from each other by large expanses of barren landscape (García et al. 2021). Among these hill formations, the “Tillandsiales” hills stand out with its unique vegetation communities specialized for living in the Atacama Desert. The dominant species of this type of formation is Tillandsia landbeckii Phil. (Bromeliaceae family), one of 15 species of the genus Tillandsia, that survive in such extremely arid conditions by developing specialized adaptations for water collection (Rundel et al. 1997; Rundel and Dillon 1998; Pinto et al. 2006; Latorre et al. 2011). Of these 15, only three species are present in the coastal Atacama Desert of northern Chile: T. landbeckii, T. marconae Till & Vitek and rarely T. virescens and T. capillaris Ruiz & Pavon (Rundel and Dillon 1998; Schulz et al. 2011; Merklinger et al. 2020). The geographic distribution of T. landbeckii extends from southern Peru to the Coquimbo region in Chile (31.65°S) (Smith and Downs 1977; Till 1992) and is restricted to the occurrence and elevation of fog belts (Oka and Ogawa 1984; Ono 1986).
Most Tillandsia plants are growing epiphytically on sandy surfaces, rocks or other plants, while some species even grow unrooted (i.e., epiarenic) on bare sand (Rundel et al. 1997; Rundel and Dillon 1998). The plants have no functional root system for water uptake therefore, they almost exclusively depend on the water and nutrients supplied by coastal fog (Pinto et al. 2006; Westbeld et al. 2009; Borthagaray et al. 2010; Gonzalez et al. 2011; Raux et al. 2020). The water uptake is carried out by highly specialized leaf trichomes unique to this plant family, which collect fog at night (Benzing et al. 1978; Rundel et al. 1997; Raux et al. 2020). The trichome of Tillandsia is formed by a shield of dead cells, with an unusually thick outer cell wall and located next to a cuticle which sits on top of the dome cell (Raux et al. 2020). The cuticle is an efficient and flexible barrier (Riederer and Schreiber 2001), constituted by cutine which is a structural component of this barrier composed of polyesters (Kolattukudy and Espelie 1985; Kunst and Samuels 2003; Jetter et al. 2006) and waxes embedded in the cutine. The water permeability of the cuticle determines the minimal and unavoidable water loss when the stomata are maximally closed during dry periods (Schuster et al. 2016). This physiological adaptation to extreme water uptake efficiency is accompanied by assimilation of carbon using Crassulacean Acid Metabolism (CAM) (Benzing et al. 1978). CAM metabolism is distinguished by the night time fixation of CO2 into malic acid allowing stomata to open only at nights when humidity is higher (Osmond et al. 1989; Haslam et al. 2003). Although CAM or a flexible C3–CAM mode of carbon fixation is known from epiphytic Tillandsia species of the coastal desert region (Rundel and Dillon 1998), foliar carbon isotope (δ13C) values of Tillandsia landbeckii of − 12.5 to − 14.3‰ (Latorre et al. 2011; Jaeschke et al. 2019) are in the typical narrow range of values corresponding to strong CAM species of bromeliads (Hermida-Carrera et al. 2020).
Leaf waxes of higher terrestrial plants are lipids that are insoluble in water (Bianchi and Canuel 2011) and contain a mixture of homologous series of long chain (> 23 carbon atoms) compounds such as fatty acids, n-alkanes, alcohols and aldehydes (Kunst and Samuels 2003; Koch and Ensikat 2008; Tafolla-Arellano et al. 2013). These leaf waxes play an important role in plant physiology and ecology, being the first zone of protection, contact and interaction with other organisms, e.g., herbivores (Müller and Riederer 2005). Leaf waxes also have the function of limiting water loss through the cuticle, minimizing the exchange of gases (e.g., CO2, O2) and water (H2O), acting as a thermoregulator barrier (Jeffree 2006; Jetter et al. 2006). Waxes also minimize the wettability and retention of dust particles and spores, and act as shield against insects and UV radiation (Tafolla-Arellano et al. 2013). The n-alkyl waxes such as fatty acids and long-chain alkanes can be characterized by their dominant homologue, total abundance, and distribution (Eglinton and Hamilton 1967; Sharma et al. 2018; Dragota and Riederer 2009). The most common indices to evaluate distribution changes of these leaf waxes are the Carbon Preference Index (CPI), which measures the relative abundance of chain length with even over odd carbon dominance in long chain fatty acids or odd over even in n-alkanes (Eglinton and Hamilton 1967; Sachse et al. 2006) and the average chain length index (ACL). Plant wax n-alkanes and fatty acids occur in modern and fossil leaves. Both are stable and long-lived molecules that can survive in the fossil record and thus serve as valuable biomarkers for past environmental conditions potentially encompassing thousands or even millions of years in soils and sediments, respectively (Eglinton and Eglinton 2008; Bush and McInerney 2013; Diefendorf and Freimuth 2017; Finstad et al. 2018).
Previous investigations of Tillandsia plants in northern Chile have studied the geographic distribution, genetic diversity, and the variation of stable carbon and nitrogen isotopes in their tissues reflecting the variability of water and nutrient supply (Pinto et al. 2006; González et al. 2011; Latorre et al. 2011; Jaeschke et al. 2019; Koch et al. 2020; Merklinger et al. 2020). However, there are currently no reports on the specific composition of leaf waxes (fatty acids and n-alkanes) in these plants. The abundance and distribution of fatty acids and n-alkanes are important chemometric and environmental indicators, since according to their changes in abundance and distribution, changes in water availability, water stress and taxonomic prevalence can be inferred (Bush and McInerney 2013, 2015; Jaeschke et al. 2018; Andrae et al. 2019; Struck et al. 2020; Mörchen et al. 2021; Ruiz-Ruiz et al. 2021). In addition, knowing the detailed composition of these waxes is the first step to more complex analyses such as compound-specific carbon, hydrogen and nitrogen isotope analyses, which can provide additional information of their photosynthetic metabolism and origin of their water source, respectively.
This study quantifies and compares the abundance and distribution (CPI and ACL) of leaf waxes, specifically long chain n-fatty acids (C24–C34) and n-alkanes (C23–C33) from individuals of Tillandsia landbeckii sampled along the coastal hyperarid core of Atacama Desert (18.47–21.21°S), and correlating with geographic (latitude, elevation, distance inland) and environmental (precipitation, temperature, surface evaporation) characteristics. Our comprehensive survey of variation among Tillandsia-specific leaf waxes will provide new insights into unique adaptation strategies for life at the dry limit.
Material and methods
Study area and sampling strategy
The northwestern coast of South America is characterized by low rainfall and a coastal desert spanning from Peru to northern Chile, politically and geographically separated in Sechura Desert (Peruvian coast between Piura and Tacna) and Atacama Desert (Contreras et al. 2010). The Atacama Desert extends west of the central Andes between 15 and 30°S across southern Peru and northern Chile (Houston and Hartley 2003). The coastal Cordillera in northern Chile with elevations of approximately 1000–1600 m a.s.l. separates the narrow coastal plain from the broad Central Valley. The study area is located in the coastal mountain range of the Chilean Atacama Desert (Fig. 1). The climate is generally mild with uniform annual air temperature of 18.9 °C in Arica and 18.4 °C in Iquique (Schulz et al. 2011). Annual precipitation is extremely low (< 2 mm; Schulz et al. 2011; Reyers et al. 2021) and rainfall is largely restricted to austral winter (Houston 2006). Summer precipitation becomes more dominant in the northern Andean zone accounting for ca. 30% of annual rainfall at Arica (Houston 2006; Schulz et al. 2011). Fog occurs frequently along the coastal Cordillera when the massive marine Stratocumulus (Sc) cloud deck that covers a large portion of the subtropical southeast Pacific approaches the coastal cliff (Cereceda et al. 2008; Schween et al. 2020; Del Río et al. 2021). Advection of marine Sc air masses facilitate fog formation in the morning and evening/night, while changes in air circulation during the afternoon produce thermal stratification that dissipates fog (Muñoz et al. 2016; Lobos Roco et al. 2018; Schween et al. 2020). The maximum spatial extent of fog typically occurs during the winter months from June to August (Del Río et al. 2018; Schween et al. 2020).
We examined nine of the largest Tillandsia ecosystems (Rundel et al., 1997; Pinto et al. 2006; Latorre et al. 2011; Koch et al. 2019) located in the coastal mountains at elevations of 900–1200 m a.s.l. between Arica and the Rio Loa Canyon (ca. 18.5–21.5°S) in March 2017 (Jaeschke et al. 2019). Tillandsia landbeckii is the predominant species, but occasionally, T. virescens, T. capillaris and T. marconae coexist, in particular toward the northern range of occurrence close to the border with Peru (Rundel et al. 1997; Pinto et al. 2006; Merklinger et al. 2020). The communities are located at distances to the coast ranging from 3 km at site 8 (Cerro Chipana) to about 27 km at site 9 located at Quebrada Rio Loa (Fig. 1; Table 1). They form either dense units or sparse and isolated stands on W and SW facing slopes directly exposed to fog. Stem and leaf tissue from multiple specimens were collected from each Tillandsia landbeckii population to cover a range in elevation and assess natural heterogeneity (Jaeschke et al. 2019). Samples were stored in geochemical bags and allowed to air-dry before shipping to Germany.
Leaf wax analysis
Plant material was first rinsed with deionized water to remove surficial mineral dust and particles and then dried in a convection oven at 40 °C. Leaves and stems of each plant sample were ground and homogenized. Bulk plant material of 59 samples (ca. 1 g) was ultrasonically extracted using a mixture of dichloromethane (DCM) and methanol (2:1, v/v), three times repeated. The extracts were combined and the bulk of the solvent subsequently removed by rotary evaporation under vacuum. The resulting total lipid extracts (TLE) were separated into apolar (with n-hexane), and polar (with DCM:methanol; 1:1, v/v) fractions using activated silica gel chromatography. The apolar fractions containing n-alkanes were further separated into saturated and unsaturated compounds over a small column filled with AgNO3-coated silica gel (10% w/w) and n-hexane and DCM, respectively. The polar fraction was separated into fatty acids and neutrals over a aminopropyl column with glacial acetic:diethyl-ether (1/24, v/v) and methanol, respectively. The fatty acid fractions were methylated with 500 µL of boron trifluoride in methanol, generating fatty acid methyl esters (FAMEs) prior to instrumental analysis. n-Alkanes were analyzed at the University of Cologne using a gas chromatograph equipped with an on-column injector and a flame ionization detector (GC-FID; HP 5890 series II) and fatty acids were analyzed at the Universidad Católica de la Santísima Concepción using a gas chromatograph with a Split/Splitless injector and a flame ionization detector (GC-FID 7890A; Agilent Technologies).
n-Alkane and fatty acid indices
The carbon preference index (CPI) was calculated using the abundances of odd- and even-numbered chain lengths from C23 to C34 for n-alkanes and fatty acids as follows:
The average chain length (ACL) of n-alkanes was calculated as follows:
where Cn is the abundance of each n-alkane or fatty acid with n carbon atoms for chain lengths from C23 to C34.
Environmental data
Estimates on mean annual precipitation and actual surface evaporation are derived from a long-term simulation with the Weather Research and Forecasting (WRF) regional climate model v3.9 (Skamarock et al. 2008). The simulation covers the period 1982 to 2017 using actual atmospheric conditions from the ERA-Interim reanalysis dataset (Dee et al. 2011) as boundary conditions and a horizontal resolution of 10 × 10 km is obtained using a double one-way nesting. A detailed description of the model setup, including details to the applied parameterizations, is given in Reyers et al. (2021). WRF simulated precipitation is validated against station measurements, and it shows good agreement with the rainfall observations particularly for the hyperarid core of the Atacama Desert (Reyers et al. 2021). The model output has an hourly temporal resolution, such that different statistics can be derived, including climatological means as used in this study. For each Tillandsia location on land, the value from the nearest grid cell was considered for comparison. Simulated daily accumulated climate data are freely available (Reyers 2019).
Statistical analysis
As the distribution of the data was not normal, we applied a Kruskal–Wallis test (non-parametric ANOVA) to estimate differences between leaf waxes for geographic zones, abundance and distribution (CPI and ACL) per zones. A Dunn’s test was used to determine whether or not there is a statistically significant difference between the medians of the three zones. To assess the effect of environmental and geographic parameters on the abundance and distribution of the leaf wax compounds, we used a Spearman’s correlation. The software Statistica 10.0.228.2 was used for statistical analysis.
Results and discussion
Leaf wax abundance
The leaf wax class dominant in Tillandsia landbeckii are n-alkanes (Fig. 2a) with an average concentration three times higher than fatty acids considering all the individuals analyzed (n = 59). The highest difference among leaf wax classes was found in the Central Zone reaching n-alkane abundances almost four times higher than fatty acids. There are few studies evaluating the composition of different leaf wax classes from plants in South America, mainly restricted to tropical areas (Jansen et al. 2006; Feakins et al. 2016) and temperate forests (Dodd et al. 1998; Rafii and Dodd 1998; Cifuentes et al. 2020; Cerda-Peña et al. 2020), where long chain fatty acids were mostly dominant compared with n-alkanes (Fig. S1). A higher concentration of n-alkanes in leaf waxes might confer to Tillandsia landbeckii individuals a more hydrophobic wax layer to avoid the loss of water from the leaves. In fact, a predominance of leaf wax n-alkanes compared with leaf wax fatty acids had been reported in two species of plants living in the Gobi Desert, located between Mongolia and China (Xu et al. 2020). The n-alkanes are produced metabolically from the fatty acids (Kolattukudy, 1966) and are the most effective wax constituents in reducing the permeability of cuticule barriers (Kosma et al. 2009; Xu et al. 2016). Therefore, the Tillandsia plants could synthesize higher amounts of n-alkanes to minimize water loss as an adaptation to extreme drought stress (Xu et al. 2020).
The dominance of n-alkanes is also visible in all three sampling zones (Fig. 2b) with the highest difference (almost 4 times higher) between average amount of both waxes in the Central Zone. The average abundance of leaf wax fatty acids was 77.1 µg/gdl with a range between 21.4 and 176.9 µg/gdl in the Northern Zone, an average of 49.5 µg/gdl with a range between 10.9 and 117.6 µg/gdl in the Central Zone, and in the Southern Zone 65.7 µg/gdl with a range between 10.2 and 152.9 µg/gdl (Fig. 2b). The average abundance of leaf wax n-alkanes in the Northern Zone was 166.5 µg/gdl with a range between 80.1 and 305.8 µg/gdl, the Central Zone hold an average of 193.8 µg/gdl ranging between 118.9 and 329.0 µg/gdl, and Southern Zone had 160.2 µg/gdl on average ranging between 10.6 and 368.3 µg/gdl (Fig. 2b). The statistical analysis (Kruskal–Wallis) did not give any significant difference among zones considering both leaf waxes (for fatty acids H = 3.2136, p = 0.2005; for n-alkanes H = 1.0413, p = 0.5941). The high variability in leaf wax abundance within a specific Tillandsia system most likely reflects the natural variability related to fine-scale changes in moisture supply and thus plant growth and fitness which was also observed in the Oyarbide field (Koch et al. 2019, 2020). In addition, age differences may also account for part of the variability as we analyzed not only the youngest leaves but the whole plant including leaves and stems.
The total amount of n-alkanes stored in Tillandsia landbeckii is in the range of those reported for CAM plants of southwest Africa (Boom et al. 2014) and shrub vegetation (i.e., Poaceae, Cyperaceae, Artemisia spp.) of the Gobi Desert (Struck et al. 2020). Concentrations are however about 10–30 times lower compared to C3 shrub vegetation analyzed in the Paposo region of Chile at ~ 24.5°S, which is also affected by fog and slightly higher rainfall amount (Mörchen et al. 2021). This may relate to plant species-specific (i.e., CAM vs. C3 photosynthetic pathway) differences in leaf size and leaf wax production, density of stomata vs trichomes covering leaves, water use efficiency and drought tolerance. In addition, C3 plants can benefit from soil moisture which is in general not available for rootless Tillandsia plants (Jaeschke et al. 2019).
Leaf wax distribution
Leaf wax fatty acid CPI values ranged between 2.5 and 11.1 with significant differences between zones (H = 20.047, p = 0.0000). CPI values were considerably lower in the Northern Zone (average = 5.1) than in the Central Zone (6.6) and Southern Zone average CPI (6.9) (Fig. 3a). CPI values of n-alkanes were less variable than those of fatty acids ranging between 6.9 and 11.3 with no significant differences between zones (H = 2.1090, p = 0.3484) and averages in Northern Zone of 8.1, in the Central Zone of 8.3, and in the Southern Zone of 8.9 (Fig. 3b).
Tillandsia landbeckii is characterized by an almost homogenous distribution of the different homologues ranging between C23–C31 (n-alkanes) and C24–C32 (fatty acids) (Fig. 4). ACL values of fatty acids range between 24.6 and 30.2 with no significant differences among zones (H = 7.2309, p = 0.0269). The ACL mean for the Northern Zone was 28.7, for the Central Zone 26.9, and for the Southern Zone 27.7 (Fig. 3c). Generally, a lower range in ACL values was observed for n-alkanes ranging from 26.0 to 28.3 (Fig. 3d), compared to fatty acid ACL values. Significant differences were found between the Northern Zone (26.7 ACL average) compared to the Central and Southern Zones (27.3 and 27.1 ACL average, respectively). The n-alkane pattern of Tillandsia landbeckii was markedly different compared to those reported for the shrub vegetation in the Atacama Desert (Mörchen et al. 2021), the Gobi Desert (Struck et al. 2020) or for CAM plants from arid southwest Africa (Boom et al. 2014), and ACL values are below those reported for C3 or C4 plants compiled by Diefendorf and Freimuth (2017). A study by Feakins and Sessions (2010) also showed a tendency to longer chain lengths in drought-adapted succulent species peaking at n–C29 to n–C33 and extreme differences in abundance and distribution of the individual n-alkane homologues. Mörchen et al. (2021) proposed that fog-affected plants can be distinguished from rain-affected plants in the Atacama Desert by their specific n-alkane pattern dominated by n–C31. We cannot confirm these observations and suggest that the distinct n-alkane distribution in Tillandsia is rather typical for this plant type using CAM metabolism in this dry and relatively cold coastal desert (Table 1).
Environmental influence on leaf wax abundance and distribution
Environmental parameters such as rainfall, temperature and relative humidity can affect the composition of higher plant leaf wax (Sachse et al. 2006; Hoffmann et al. 2013; Bush and McInerney 2015; Jaeschke et al. 2018; Andrae et al. 2019). Because of the high intra-system variability of Tillandsia plant waxes described above, we use site-average abundances to better constrain relationships with climate parameters along the investigated coastal transect (Jaeschke et al. 2019).
The Mean Annual Precipitation (MAP) derived from the WRF simulation (Reyers 2019; Jaeschke et al. 2019) is extremely low at the study sites (≤1.5 mm/yr). However, the highest significant Spearman's Rank correlation coefficient was observed between latitude and MAP (rs = − 0.726; p < 0.001, Table 2), showing a significant decrease in moisture content across the investigated transect. It’s well known that the main source of moisture at the western coast of Northern South America is associated to the seasonal migration of the Intertropical Convergence Zone (Takahashi and Battisti 2007). However, latitude correlates significantly and negatively also with actual surface evaporation but to a lower degree (rs = − 0.417; p < 0.001). It is important to remember that we are talking at very low levels and scarce (infrequent) precipitation, thus fog must play a significant role as moisture source (Lobos-Roco et al. 2018; Schween et al. 2020; Böhm et al. 2021; Del Rio et al. 2021; García et al. 2021). A significant and positive trend was observed between precipitation and elevation (rs = 0.451; p < 0.001, Table 2), which may relate to the higher water content at the top of the Sc cloud deck (Cereceda et al. 2008; Latorre et al. 2011; Jaeschke et al. 2019). However, neither water content of the Sc cloud or fog are included in the WRF precipitation, which may explain some of the variability seen in our data. A promising fog-detecting approach was recently introduced by Böhm et al. (2021) and may help to disentangle the influence of different moisture sources on Tillandsia lomas.
The abundance of leaf wax fatty acids did not show significant correlations with any of the climate variables. However, the n-alkane abundance correlates significantly and positively with ACL of n-alkanes (rs = 0.413; p < 0.001) and negatively with surface evaporation (rs = − 0.261; p < 0.05). Therefore, increasing abundance of n-alkanes are related with an increase in abundance of longer chain length of n-alkanes. In addition, the low but significant correlation with surface evaporation suggests an increase of n-alkanes at low moisture conditions. CPI of fatty acids correlates significantly with many variables but the highest correlations were observed with latitude and precipitation (rs = 0.580; p < 0.001 and − 0.562; p < 0.001, respectively), showing a clear response to variable moisture content (Table 2; Fig. 5). A high CPI is the main characteristic of leaf waxes, which must be related to leaf wax production that is not visible in the fatty acid abundance. The highest correlation of n-alkane CPI values was observed with distance to the coast (rs > 0.4; p < 0.001, Table 2). The ACL of the fatty acids did not show high significant correlation (Table 2) but ACL of the n-alkanes correlates negatively with both, precipitation (rs = − 0.416; p < 0.001) and surface evaporation (rs = − 0.485; p < 0.001) (Fig. 5). The latter correlation, where lower moisture is related to higher abundance of longer carbon chain length of n-alkanes, confirm a direct response of the leaf wax distribution (ACL and CPI) to moisture gradients. Aridity has been proposed as a driver of n-alkane chain length, where drier conditions promote generally longer chain lengths (Dodd et al. 1998; Feakins and Sessions 2010; Hoffmann et al. 2013; Andrae et al. 2019). It is thought that under arid and usually warm conditions, selective pressures may favor the production of longer, more hydrophobic n-alkane chain lengths (Shepherd and Griffiths 2006; Bush and McInerney 2013). However, opposite trends between ACL and aridity have also been found in leaves and soils (Hoffmann et al. 2013; Carr et al. 2014; Jaeschke et al. 2018). The observed dissimilarities may thus relate to genetic or taxonomic differences besides being solely modulated by environmental parameters (Bush and McInerney 2013; Carr et al. 2014; Andrae et al. 2019; Xu et al. 2020).
Leaf waxes to understand moisture at the dry limits of the hyperarid Atacama Desert
Saturated long-chain (> C20) fatty acids are the precursors for the biosynthesis of aliphatic cuticular wax components, such as primary and secondary alcohols, aldehydes, alkanes, ketones and alkyl esters (Kolattukudy 1966). In fact, Kolattukudy (1966, 1967) proposed an elongation–decarboxylation pathway for alkane biosynthesis. During this pathway, the shorter chain fatty acids (< C20) are elongated by the stepwise addition of C2 units followed by decarboxylation until an appropriate length (C30–C32) is reached. The resulting leaf wax n-alkanes then show the typical odd over even dominance (Kunst et al. 2006). The leaf wax trends observed in this study (Table 2) are related to leaf wax production under extremely low moisture conditions where CPI of fatty acids and ACL of n-alkanes correlate with moisture availability (Fig. 5). More studies are required to understand the full mechanisms behind this strong relationship with moisture for plants thriving at the dry limit.
Conclusions
This study investigated leaf wax n-alkane and fatty acid distributions in modern Tillandsia landbeckii plants located in isolated patches along the Coastal Cordillera of the Atacama Desert to investigate their potential as chemotaxonomic markers and dependency on climate. Our results show that Tillandsia plants produce more n-alkanes than fatty acids in their leaves likely as an expression of enhanced drought tolerance. While n-alkane and fatty acid concentrations and chain-length patterns are highly variable within a specific ecosystem, likely reflecting small-scale changes in moisture availability and natural variability, site-averaged values show significant correlations with annual precipitation and evaporation along the investigated coastal transect. Our results indicate sensitive changes in leaf wax composition of Tillandsia to subtle differences in climate parameters pointing to an adaptive strategy to the extremely arid conditions at the molecular level. The Tillandsia-specific chemical fingerprints can thus provide crucial information on past climate variability in the Atacama Desert.
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Acknowledgements
This study was part of the CRC 1211 ‘Earth – Evolution at the dry limit’ supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Grant 268236062 – SFB 1211. SC and ML acknowledge the grant ANID FONDECYT 1190398. We thank F. McInerney and M. Koch for their valuable comments improving the quality of this manuscript.
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SC and AJ conceived and designed the project. AJ conducted the field work. ML, KG, SC and AJ analyzed the samples. MR provided WRF model data. SC and AJ drafted the manuscript. CL, MR, KG and JR reviewed the manuscript. All authors contributed to the final manuscript draft.
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Online Resource 1. Comparison of leaf wax fatty acids and n-alkanes of Tillandsia landbeckii with those of two trees from a temperate forest in Chile.
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Contreras, S., Landahur, M., García, K. et al. Leaf wax composition and distribution of Tillandsia landbeckii reflects moisture gradient across the hyperarid Atacama Desert. Plant Syst Evol 308, 8 (2022). https://doi.org/10.1007/s00606-021-01800-0
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DOI: https://doi.org/10.1007/s00606-021-01800-0