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Molecular characterization of sawtooth oak (Quercus acutissima) germplasm based on randomly amplified polymorphic DNA

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Abstract

Sawtooth oak (Quercus acutissima) is a predominant tree species in the deciduous broad-leaved forest in China. It distributes in a large landscape area and can disperse in various ecology types. Molecular study on sawtooth oak can provide valuable information about the genetic diversity level and genetic relatedness on this important tree species. Insight into the genetic structure also provides resources of a species with its current feature and future evolutionary potential. The genetic structure of sawtooth oak was investigated by randomly amplified polymorphic DNA (RAPD). Twelve RAPD markers were used to assess genetic diversity of 408 individuals from 17 provenances enveloping most of the current distribution area of sawtooth oak. A total of 66 amplification products were detected, of which 49 bands (74.24 %) were polymorphic. Nei’s gene diversity, 0.2409, indicated a relatively high level of genetic variation in sawtooth oak germplasm. Analysis of molecular variance showed that most of the genetic diversity (87 %) was allocated within provenances. A combination of UPGMA dendrogram and STRUCTURE analysis was employed to estimate the genetic relationships of sawtooth oak germplasm; interestingly, the two methods presented similar grouping pattern with few discrepancies. Results revealed that 16 out of 17 provenances were clustered into one group, while the other 1 (LQ provenance) constituted a separate cluster. The data presented in this study suggested that the RAPD method was a valuable tool for estimation of genetic diversity and genetic relatedness of sawtooth oak germplasm. The present study also gave useful implications for germplasm conservation and new cultivar development for this promising energy tree species.

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Acknowledgments

We owe special thanks to Dr. ZL Liu for her previous work on collecting acorns of sawtooth oak. We would also like to acknowledge Y Xia and YX Chen for their contributions in collecting samples. This work was supported by the project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), the Doctorate Fellowship Foundation of Nanjing Forestry University and the National Key Technology R & D Program (2008BAC39B01, 2008BAC39B06).

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Authors and Affiliations

  1. College of Forest Resources and Environment, Nanjing Forestry University, 159, Longpan Rd, Nanjing, 210037, People’s Republic of China

    Yuan-Yan Zhang, Yan-Ming Fang & Xiao-Li Zhou

  2. Research Institute of Subtropical Forestry, Fuyang, 311400, Zhejiang, People’s Republic of China

    Mu-Kui Yu

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  1. Yuan-Yan Zhang
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  2. Yan-Ming Fang
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Correspondence to Yan-Ming Fang.

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Results of Mantel test based on RAPD data of sawtooth oak

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Zhang, YY., Fang, YM., Yu, MK. et al. Molecular characterization of sawtooth oak (Quercus acutissima) germplasm based on randomly amplified polymorphic DNA. Plant Syst Evol 299, 1829–1837 (2013). https://doi.org/10.1007/s00606-013-0838-2

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  • DOI: https://doi.org/10.1007/s00606-013-0838-2

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