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Use of genomic and genic SSR markers for assessing genetic diversity and population structure in Indian and African finger millet (Eleusine coracana (L.) Gaertn.) germplasm

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Abstract

Genetic diversity and population structure were analyzed in 67 diverse finger millet accessions of African and Indian origin. A total of 69 alleles were generated with a mean of 4.0 alleles per locus and with an average gene diversity of 0.471. Molecular diversity parameters showed higher values in African accessions. Nineteen rare and nine unique alleles were observed and the identified accessions can be a potential source for further improvement of finger millet. Clustering of south Indian accessions with African lowland types and north/highland Indian accessions with that of African highlands was also observed. Structure analysis revealed the distinctness of Ugandan accessions compared to other African accessions and five major sub-populations useful for parental selection, conservation, and utilization of finger millet.

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References

  • Arya L, Verma M, Gupta VK et al (2009) Development of EST-SSRs in finger millet (Eleusine coracana ssp. coracana) and their transferability to pearl millet (Pennisetum glaucum). J Plant Biochem Biotechnol 18:97–100

    Article  CAS  Google Scholar 

  • Babu BK, Senthil N, Gomez SM et al (2007) Assessment of genetic diversity among finger millet (Eleusine coracana (L.) Gaertn.) accessions using molecular markers. Genet Resour Crop Ev 54:399–404

    Article  CAS  Google Scholar 

  • Barbeau WE, Hilu KW (1993) Protein, calcium, iron and amino acid content of selected wild and domesticated cultivars of finger millet. Plant Foods Hum Nutr 43:97–104

    Article  PubMed  CAS  Google Scholar 

  • Dida MM, Srinivasachary, Ramakrishnan S et al (2007) The genetic map of finger millet, Eleusine coracana. Theor Appl Genet 114:321–332

    Article  PubMed  CAS  Google Scholar 

  • Dida MM, Wanyera N, Harrison Dunn ML et al (2008) Population structure and diversity in finger millet (Eleusine coracana) germplasm. Trop Plant Biol 1:131–141

    Article  Google Scholar 

  • Fakrudin B, Shashidhar HE, Kulkarni RS et al (2004) Genetic diversity assessment of finger millet (Eleusine coracana Gaertn.), germplasm through RAPD analysis. PGR Newslett 138:50–54

    Google Scholar 

  • Hilu KW (1995) Evolution of finger millet: evidence from random amplified polymorphic DNA. Genome 38:232–238

    Article  PubMed  CAS  Google Scholar 

  • Hilu KW, de Wet JMJ (1976) Domestication of Eleusine coracana. Econ Bot 30:199–208

    Article  Google Scholar 

  • Liu K, Muse SV (2005) PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics 21:2128–2129

    Article  PubMed  CAS  Google Scholar 

  • Nei M (1973) Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci USA 70:3321–3323

    Article  PubMed  CAS  Google Scholar 

  • Panwar P, Saini RK, Sharma N et al (2010) Efficiency of RAPD, SSR and cytochrome P450 gene based markers in accessing genetic variability amongst finger millet (Eleusine coracana) accessions. Mol Biol Rep 37(8):4075–4082

    Article  PubMed  CAS  Google Scholar 

  • Powell W, Morgante M, Andre C et al (1996) The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol Breed 2:225–238

    Article  CAS  Google Scholar 

  • Prasada Rao KE, de Wet JMJ (1997) Small millets. In: Fuccillo D, Sears L, Stapleton P (eds) Biodiversity in trust. Cambridge University Press, UK, pp 259–272

    Google Scholar 

  • Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959

    PubMed  CAS  Google Scholar 

  • Rohlf FJ (1993) NTSYSpc numerical taxonomy and multivariate analysis system, version 2.1. Exeter Publishing Ltd., NY

    Google Scholar 

  • Saghai-Maroof MA, Soliman KM, Jorgensen RA et al (1984) Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location and population dynamics. Proc Natl Acad Sci 81:8014–8018

    Article  PubMed  CAS  Google Scholar 

  • Salimath SS, de Oliveira AC, Godwin ID et al (1995) Assessment of genome origins and genetic diversity in the genus Eleusine with DNA markers. Genome 38:757–763

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

Authors gratefully acknowledge Indian Council of Agricultural Research (ICAR) for financial support of this work. We further express our special thanks to Project Coordinator, All India Coordinated Small Millet Improvement Project (AICSMIP), Bangalore and Dr. Sunil Archak, Senior Scientist, NRC on DNAFP, NBPGR for providing seeds of finger millet for the present study. We are also thankful to Director, NBPGR, New Delhi for providing facilities for this work.

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Authors and Affiliations

  1. National Research Centre on DNA Fingerprinting, National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, 110012, India

    L. Arya & M. Verma

  2. Department of Biochemistry, Kurukshetra University, Kurukshetra, 136119, India

    V. K. Gupta

  3. All India Coordinated Small Millet Improvement Project (AICSMIP), Bangalore, 560065, India

    A. Seetharam

Authors
  1. L. Arya
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  2. M. Verma
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  3. V. K. Gupta
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  4. A. Seetharam
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Correspondence to L. Arya.

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Arya, L., Verma, M., Gupta, V.K. et al. Use of genomic and genic SSR markers for assessing genetic diversity and population structure in Indian and African finger millet (Eleusine coracana (L.) Gaertn.) germplasm. Plant Syst Evol 299, 1395–1401 (2013). https://doi.org/10.1007/s00606-013-0822-x

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  • DOI: https://doi.org/10.1007/s00606-013-0822-x

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