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Cytogenetic and flow cytometry data expand knowledge of genome evolution in three Coffea species

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Abstract

Karyotype and nuclear 2C-value data are considered important in taxonomic and evolutionary approaches in Coffea. Still, new methods are needed to further support such studies, especially to determine the progenitors of Coffea arabica. In this work, new cytogenetic and flow cytometry data were used to compare Coffea arabica, Coffea canephora and Coffea congensis. These data corroborate the hypothesis that C. canephora and C. congensis originated from a single ancestor, whose basic chromosome number was x = 11. In agreement with the observations of other authors, the karyotype and mean 2C-values confirm that C. arabica is a true allotetraploid originating from two diploid Coffea species with similar genomes. Although C. canephora and C. congensis have been considered potential progenitors of C. arabica, karyotype comparison revealed that only one of these species may be parental to C. arabica. These accurate cytogenetic and flow cytometry data contribute to expand our knowledge of the Coffea genome, as well as of possible progenitors of C. arabica.

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Acknowledgments

We thank FAPES (Fundação de Amparo à Pesquisa do Espírito Santo), FAPEMIG (Fundação de Amparo à Pesquisa de Minas Gerais), CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), CBP&D/Café (Consórcio Brasileiro de Pesquisa e Desenvolvimento do Café) and CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior), Brazil, for their financial support. We also thank BioCafé/BIOAGRO (Viçosa-MG, Brazil) for their support, especially to Dr. Eveline Teixeira Caixeta for her technical assistance and advice.

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  1. Laboratório de Microscopia, Departamento de Produção Vegetal, Centro de Ciências Agrárias, Universidade Federal do Espírito Santo, Alegre, ES, 29500-000, Brazil

    Wellington Ronildo Clarindo

  2. Laboratório de Citogenética e Citometria, Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil

    Carlos Roberto Carvalho & Maria Andréia Corrêa Mendonça

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  1. Wellington Ronildo Clarindo
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  2. Carlos Roberto Carvalho
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  3. Maria Andréia Corrêa Mendonça
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Correspondence to Carlos Roberto Carvalho.

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Clarindo, W.R., Carvalho, C.R. & Mendonça, M.A.C. Cytogenetic and flow cytometry data expand knowledge of genome evolution in three Coffea species. Plant Syst Evol 298, 835–844 (2012). https://doi.org/10.1007/s00606-012-0595-7

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