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Selective adsorption and identification of penicillin G sodium in milk by molecularly imprinted polymer doped carbon dot

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Abstract

A carbon dot (CD) was prepared by o-phenylenediamine and water, which showed bright yellow fluorescence under ultraviolet light irradiation (λ = 580 nm), and verified good fluorescence quenching effect on penicillin G sodium (Png-Na). Using methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a crosslinker, and Png-Na as a template, a kind of composite microsphere combining CD and molecularly imprinted polymer (MIP) was synthesized by surface-initiated atomic transfer radical polymerization (SI-ATRP). For reasons of comparison, we also prepared MIP without CD and non-imprinted polymers (NIPs). Through static and dynamic adsorption experiments, the maximum adsorption capacity was 47.05 mg g−1 and the equilibrium time was 30 min. High-performance liquid chromatography (HPLC) was utilized to determine the content of Png-Na in the spiked milk samples. A sensitive, rapid, and simple method for determination of Png-Na in food samples was developed. The utilized approach enabled the quantification of Png-Na within the concentration range 20–1000 μg L−1 (with a limit of detection of 5 μg L−1). The recoveries achieved were in the range 93.3–98.2%, with a relative standard deviation of 1.2–4.2%. The results demonstrated that CD@MIP possessed the capability of specific adsorption and fluorescence detection of Png-Na, enabling simultaneous detection and enrichment of Png-Na in real samples.

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Acknowledgements

Financial support is gratefully acknowledged from the CAS-Weigao Research & Development Program ([2017]-009) and the National Natural Sciences Foundation of Ningxia (No. 2021AAC02017) to J. Ou.

Funding

This work was supported by National Natural Sciences Foundation of Ningxia, No. 2021AAC02017, Junjie Ou, The CAS-Weigao Research & Development Program, [2017]-009, Junjie Ou.

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Key Laboratory for Chemical Engineering and Technology, State Ethnic Affairs Commission, North Minzu University, Yinchuan, 750021, China

    Yi Xu, Haiping Wang, Qingyan Bai & Chunmiao Bo

  2. College of Chemistry and Materials Science, Northwest University, Xi’an, 710127, China

    Junjie Ou

  3. CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China

    Shujuan Ma & Junjie Ou

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  1. Yi Xu
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  2. Haiping Wang
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  3. Qingyan Bai
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Correspondence to Chunmiao Bo or Junjie Ou.

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Xu, Y., Wang, H., Bai, Q. et al. Selective adsorption and identification of penicillin G sodium in milk by molecularly imprinted polymer doped carbon dot. Microchim Acta 191, 186 (2024). https://doi.org/10.1007/s00604-024-06269-5

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