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An impedance labeling free electrochemical aptamer sensor based on tetrahedral DNA nanostructures for doxorubicin determination

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Abstract

Based on the electrochemical impedance method, a marker-free biosensor with aptamer as a biometric element was developed for the determination of doxorubicin (DOX). By combining aptamer with rigid tetrahedral DNA nanostructures (TDNs) and fixing them on the surface of gold electrode (GE) as biometric elements, the density and directivity of surface nanoprobes improved, and DOX was captured with high sensitivity and specificity. DOX was captured by immobilized aptamers on the GE, which inhibited electron transfer between the GE and [Fe(CN)6]3−/4− in solution, resulting in a change in electrochemical impedance. When the DOX concentration was between 10.0 and 100.0 nM, the aptasensor showed a linear relationship with charge transfer resistance, the relative standard deviation (RSD) ranged from 3.6 to 5.9%, and the detection limit (LOD) was 3.0 nM. This technique offered a successful performance for the determination of the target analyte in serum samples with recovery in the range 97.0 to 99.6% and RSD ranged from 4.8 to 6.5%. This method displayed the advantages of fast response speed, good selectivity, and simple sensor structure and showed potential application in therapeutic drug monitoring.

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Funding

This research was supported by the National Natural Science Foundation of China (No. 21675177) and the Youth Science Foundation of Guangxi Medical University (No. GXMUYSF202338).

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Authors and Affiliations

  1. Department of Pharmacy, The Second Affiliated Hospital of Guangxi Medical University, Nanning, 530007, China

    Dan Ou

  2. School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, 510006, China

    Dan Ou & Zuanguang Chen

  3. Department of Interventional Radiology, Guangxi Academy of Medical Science, The People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, 530016, China

    Huixian Yan

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  1. Dan Ou
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Ou, D., Yan, H. & Chen, Z. An impedance labeling free electrochemical aptamer sensor based on tetrahedral DNA nanostructures for doxorubicin determination. Microchim Acta 191, 94 (2024). https://doi.org/10.1007/s00604-024-06176-9

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