Abstract
A new surface-enhanced Raman spectroscopy (SERS) biosensor of Graphene@Ag-MLF composite structure has been fabricated by loading AgNPs on graphene films. The response of the biosensor is based on plasmonic sensing. The results showed that the enhancement factor of three different spores reached 107 based on the Graphene@Ag-MLF substrate. In addition, the SERS performance was stable, with good reproducibility (RSD<3%). Multivariate statistical analysis and chemometrics were used to distinguish different spores. The accumulated variance contribution rate was up to 96.35% for the top three PCs, while HCA results revealed that the spectra were differentiated completely. Based on optimal principal components, chemometrics of KNN and LS-SVM were applied to construct a model for rapid qualitative identification of different spores, of which the prediction set and training set of LS-SVM achieved 100%. Finally, based on the Graphene@Ag-MLF substrate, the LOD of three different spores was lower than 102 CFU/mL. Hence, this novel Graphene@Ag-MLF SERS substrate sensor was rapid, sensitive, and stable in detecting spores, providing strong technical support for the application of SERS technology in food safety.
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Funding
This research was supported by the Major science and technology projects in Henan province (221100110500), the Science Foundation for Outstanding Youth of Henan Province (212300410008), the Science and Technology Innovation Team of Henan Universities (22IRTSTHN021), the key scientific and technological projects of Henan Province (232102110136), the National Modern Agriculture (beef yak) Industrial Technology System Construction Special (CARS-37), and the Key R&D Project of Henan Provincial Department of Education (22A550009).
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YZ: investigation, data curation, writing—original draft. JT: writing—original draft. ML: supervision, resources, conceptualization, writing—review and editing. LZ: supervision, funding acquisition. JS: data curation. WL: investigation. SL: helped collect test data. DL: supervision. GZ: investigation. LX: writing—review and editing. SY: assisted the experiment and collected test data.
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Zhu, Y., Tian, J., Li, M. et al. Construction of Graphene@Ag-MLF composite structure SERS platform and its differentiating performance for different foodborne bacterial spores. Microchim Acta 190, 472 (2023). https://doi.org/10.1007/s00604-023-06031-3
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DOI: https://doi.org/10.1007/s00604-023-06031-3