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Dual-mode transfer response based on electrochemical and fluorescence signals for the detection of amyloid-beta oligomers (AβO)

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Abstract

An aptamer sensor has been developed utilizing a dual-mode and stimuli-responsive strategy for quantitative detection of AβO (amyloid-beta oligomers) through simultaneous electrochemical and fluorescence detection. To achieve this, we employed UIO-66-NH2 as a carrier container to load MB (Methylene Blue), and Fe3O4 MNPs (iron oxide magnetic nanoparticles) with aptamer (ssDNA-Fe3O4 MNPs) fixed on their surface for biological gating. The ssDNA-Fe3O4 MNPs were immobilized onto the surface of UIO-66-NH2 through hydrogen bonding between the aptamer and the –NH2 group on the surface of UIO-66-NH2, thereby encapsulating MB and forming ssDNA-Fe3O4@MB@UIO-66-NH2. During the detection of AβO, the aptamer selectively reacted with AβO to form the AβO-ssDNA-Fe3O4 complex, leading to its detachment from the surface of UIO-66-NH2. This detachment facilitated the release of MB, enabling its electrochemical detection. Simultaneously, the AβO-ssDNA-Fe3O4 complex was efficiently collected and separated using a magnet after leaving the container’s surface. Furthermore, the addition of NaOH facilitated the disconnection of biotin modifications at the 3′ end of the aptamer from the avidin modifications on the Fe3O4 MNPs. Consequently, the aptamer detached from the surface of Fe3O4 MNPs, resulting in the restoration of fluorescence intensity of FAM (fluorescein-5′-carboxamidite) modified at its 5′ end for fluorescence detection. The dual-mode sensor exhibited significantly enhanced differential pulse voltammetry signals and fluorescence intensity compared to those in the absence of AβO. The sensor demonstrated a wide detection range of 10 fM to 10 μM, with a detection limit of 3.4 fM. It displayed excellent performance in detecting actual samples and holds promising prospects for early diagnosis of Alzheimer’s disease.

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Funding

This research was funded by Shandong Provincial Natural Science Foundation (Grant No. ZR2019MB066) and the National Natural Science Foundation of China (Grants No. 21802052). The authors are thankful to the Deanship of Scientific Research and supervision of the Centre for Health Research at Najran University, Najran, Kingdom of Saudi Arabia, for funding under the Research Centers funding program Grant No. NU/RCP/MRC/12/2.

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Authors and Affiliations

  1. Institute of Surface Analysis and Chemical Biology, University of Jinan, Jinan, 250022, China

    Zhe Ren, Wenjuan Guo, Shuqian Sun, Xin Liu & Zelong Fan

  2. School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, China

    Fangfang Wang

  3. Department of Chemistry, College of Science and Arts, and Promising Centre for Sensors and Electronic Devices (PCSED), Najran University, Najran, 11001, Kingdom of Saudi Arabia

    Ahmed A. Ibrahim & Ahmad Umar

  4. Department of Materials Science and Engineering, The Ohio State University, Columbus, OH, 43210, USA

    Ahmad Umar

  5. Centre for Health Research, Najran University, Najran, 11001, Kingdom of Saudi Arabia

    Abdulrab Ahmed M. Alkhanjaf

  6. Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Najran University, Najran, 11001, Kingdom of Saudi Arabia

    Abdulrab Ahmed M. Alkhanjaf

  7. Department of Materials Science, University of Patras, Patras, Greece

    S. Baskoutas

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  1. Zhe Ren
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  2. Wenjuan Guo
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  4. Xin Liu
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Correspondence to Wenjuan Guo, Fangfang Wang or Ahmad Umar.

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Ren, Z., Guo, W., Sun, S. et al. Dual-mode transfer response based on electrochemical and fluorescence signals for the detection of amyloid-beta oligomers (AβO). Microchim Acta 190, 438 (2023). https://doi.org/10.1007/s00604-023-06014-4

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