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Label free dual-mode sensing platform for trace level monitoring of ciprofloxacin using bio-derived carbon dots and evaluation of its antioxidant and antimicrobial potential

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Abstract

Being a persuasive antibiotic, ciprofloxacin is widely administered to patients and its excessive discharge has generated a keen interest among researchers for its detection in water resources. Therefore, the current work utilizes the virtues of carbon dots synthesized from the leaves of Ocimum sanctum as an economical and convenient bimodal stratagem for the detection of ciprofloxacin via an electrochemical and fluorometric approach. The insight into photostability, size, morphology, and optical studies of the carbon dots was tested to enhance their scope in sensing. The excellent photoluminescence-based excitation-dependent behavior with a quantum yield of 46.7% and non-requirement of any kind of labeled surface variations for amending their fluorescence and electrochemical properties have further supported the utilization of as-prepared carbon dots in trace-level monitoring of ciprofloxacin. The fluorescence emission intensity and peak current were enhanced by many folds via the application of Ocimum sanctum–derived carbon dots. The synergetic effect of carbon dots has possessed a linear relationship between the peak current/emission intensity within the range of 0 to 250 μM of ciprofloxacin and the lowest detection limit value was found to be 0.293 and 0.0822 μM with fluorometric and electrochemical methods, respectively. The sensor demonstrated excellent applicability for the estimation of ciprofloxacin and acts as a high-performance dual sensor for further applications.

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Acknowledgements

The authors are thankful to the Deanship of Scientific Research and the supervision of the Centre for Scientific and Engineering Research at Najran University, Najran, Kingdom of Saudi Arabia, for funding under the Research Centers funding program Grant No. NU/RCP/SERC/12/3. Anjali Vijeata is thankful to Council of Scientific and Industrial Research (CSIR), New Delhi, for financial support under CSIR (09/135(0894)/2019-EMR-l). Savita Chaudhary is thankful to DST Inspire Faculty award [IFACH-17], Haryana State Council for Science and Technology [HSCSIT/R&D/2020/476], and DST Chandigarh and DST Purse grants II for financial assistance. Ganga Ram Chaudhary would like to acknowledge the support of UGC, India, under the INDO-US 21st Century knowledge Initiative project [F.No. 194-2/2016 (IC)]. The authors are thankful to the Sophisticated Analytical Instrumentation Facility (SAIF, CIL), Panjab University, Chandigarh, for the technical support.

Author contribution

Anjali Vijeata: conceptualization, methodology, software, validation, formal analysis, investigation, data curation, writing—original draft.

Savita Chaudhary: conceptualization; methodology; validation; formal analysis; resources ; writing—review and editing; visualization ; supervision ; project administration; funding acquisition.

Ahmad Umar: methodology; visualization; writing—original draft; writing—review and editing; formal analysis; resources.

Ganga Ram Chaudhary, Sheikh Akbar, Sotirios Baskoutas: methodology; visualization ; writing—review and editing; formal analysis; resources.

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Authors and Affiliations

  1. Department of Chemistry and Centre of Advanced Studies in Chemistry, Panjab University, Chandigarh, 160014, India

    Anjali Vijeata, Ganga Ram Chaudhary & Savita Chaudhary

  2. Centre for Scientific and Engineering Research, Najran University, Najran, 11001, Kingdom of Saudi Arabia

    Ahmad Umar

  3. Department of Chemistry, College of Science and Arts, and Promising Centre for Sensors and Electronic Devices (PCSED), Najran University, Najran, -11001, Kingdom of Saudi Arabia

    Ahmad Umar

  4. Department of Materials Science and Engineering, The Ohio State University, Columbus, OH, 43210, USA

    Ahmad Umar & Sheikh Akbar

  5. Department of Materials Science, University of Patras, 26504, Patras, Greece

    Sotirios Baskoutas

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  1. Anjali Vijeata
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Correspondence to Savita Chaudhary or Ahmad Umar.

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Vijeata, A., Chaudhary, G.R., Chaudhary, S. et al. Label free dual-mode sensing platform for trace level monitoring of ciprofloxacin using bio-derived carbon dots and evaluation of its antioxidant and antimicrobial potential. Microchim Acta 190, 258 (2023). https://doi.org/10.1007/s00604-023-05830-y

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