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Magnetic relaxation switch sensor based on aptamer-modified poly-L-lysine-ferroferric oxide magnetic nanoparticles and graphene oxide for the determination of insecticides in vegetables

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Abstract

A simple and effective graphene oxide-magnetic relaxation switch (GO-MRS) sensor that combines graphene oxide (GO) and aptamer-modified poly-L-lysine(PLL)-Fe3O4 nanoparticles (Fe3O4@PLL–Apt NPs) was designed for the detection of acetamiprid (ACE). In this sensor, Fe3O4@PLL–Apt NPs acted as a relaxation signal probe and GO facilitated the generation of relaxation signal changes (dispersion/aggregation shift), while the aptamer is a molecular component that recognizes ACE. This GO-assisted magnetic signal probe improves the stability of magnetic nanoparticles in solution and enhances their sensitivity to small molecules while avoiding cross-reactions. Under optimal conditions, the sensor exhibits a wide working range (10–80 nM) and low detection limit (8.43 nM). The spiked recoveries ranged from 96.54 to 103.17%, with a relative standard deviation (RSD) of less than 2.3%. In addition, the performance of the GO-MRS sensor matched that of the standard method (liquid chromatography-mass spectrometry (LC–MS)), indicating that the GO-MRS sensor is suitable for the detection of ACE in vegetables.

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Data availability

The data that support the findings of this study are available from the corresponding author, [Xin Wang], upon reasonable request.

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Funding

This work was supported by the National Natural Science Foundation of China (No. 81773482).

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Author notes

  1. Lei Huang and Kaili Pei contributed equally to this work.

Authors and Affiliations

  1. School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China

    Lei Huang, Kaili Pei & Xin Wang

Authors
  1. Lei Huang
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  2. Kaili Pei
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  3. Xin Wang
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Contributions

Lei Huang: writing—review and editing, formal analysis, software; Kaili Pei: methodology, writing—original draf; Xin Wang: validation, project administration, supervision, funding acquisition.

Corresponding author

Correspondence to Xin Wang.

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Highlights

1. A sensitive method for acetamiprid in vegetables was proposed.

2. A graphene oxide-magnetic relaxation switch (GO-MRS) sensor was designed.

3. This method has a satisfactory LOD (8.43 nM).

4. The detection of acetamiprid in real samples was agreed with LC-MS.

Supplementary Information

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604_2023_5817_MOESM1_ESM.pdf

Supplementary file1 (PDF 1637 KB) Instruments, experimental materials, detailed methods, and relevant analytical data can be found in the Electronic Supporting Material.

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Huang, L., Pei, K. & Wang, X. Magnetic relaxation switch sensor based on aptamer-modified poly-L-lysine-ferroferric oxide magnetic nanoparticles and graphene oxide for the determination of insecticides in vegetables. Microchim Acta 190, 239 (2023). https://doi.org/10.1007/s00604-023-05817-9

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