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Molecularly imprinted polymers enhanced peroxidase-like activity of AuNPs for determination of glutathione

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Abstract

An elaborate composite of molecularly imprinted polymer (MIP)-modified gold nanoparticles (AuNPs)@silica dioxide (SiO2) was designed and prepared for real-time colorimetric determination of glutathione (GSH) in serum. Firstly, the MIPs were synthesized on the surface of SiO2 utilizing GSH as template molecules. Then, AuNPs were synthesized on the surface of MIPs@SiO2 to produce a composite of MIPs modified by AuNPs@SiO2. Compared with plain AuNPs, the composite possessed better peroxidase catalysis activity due to stabilization and protection from hydrophilic SiO2, which can catalyze H2O2 to·OH oxidizing 3,3,5,5-tetramethylbenzidine (TMB) to the colored product. In addition, its selectivity was enhanced by MIP modification with special recognition cavities. With the composite as the sensor, GSH was precisely and sensitively detected in the range 5 ~ 40 μM with a limit of determination of 1.16 μM according to the principle of inhibitive peroxidase catalysis activity by GSH. The proposed colorimetric detection was successfully utilized for selective, convenient, and rapid determination of GSH in serum. It provided a new strategy for drug real-time monitoring and has high potential in clinical drug analysis.

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Funding

This research was financially supported by the Doctoral Scientific Research Foundation of Jining Medical University (No. 6001/600761003), Research Fund for Lin He’s Academician Workstation of New Medicine and Clinical Translation in Jining Medical University (JYHL2018MS16), Supporting Fund for Teachers’ Research of Jining Medical University (JYFC2019KJ029), the National Innovation and Entrepreneurship Training Program for Undergraduate (No. 202210316026Z), and the Innovation and Entrepreneurship Training Program for Undergraduate (No. 202210316316).

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Author notes

  1. Xiaoni Zhang, Jun Peng, and Liping Xi contributed equally to this work.

Authors and Affiliations

  1. Department of Chemistry, China Pharmaceutical University, Nanjing, 211198, China

    Xiaoni Zhang, Liping Xi, Ziwei Lu, Lili Yu, Meiru Liu, Dezhi Huo & Hua He

  2. College of Medical Engineering & the Key Laboratory for Medical Functional Nanomaterials, Jining Medical University, Jining, 272067, China

    Jun Peng

  3. Key Laboratory of Biomedical Functional Materials, China Pharmaceutical University, Nanjing, 211198, China

    Hua He

  4. Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, China Pharmaceutical University, 639 Longmian Avenue, NanjingJiangsu Province, 211198, China

    Hua He

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  1. Xiaoni Zhang
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Contributions

Conceptualization: Xiaoni Zhang, methodology: Jun Peng, formal analysis and investigation: Liping Xi, writing—original draft preparation: Ziwei Lu, writing—review and editing: LiLi Yu, funding acquisition: Meiru Liu, resources: Dezhi Huo, and supervision: Hua He.

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Correspondence to Jun Peng or Hua He.

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Zhang, X., Peng, J., Xi, L. et al. Molecularly imprinted polymers enhanced peroxidase-like activity of AuNPs for determination of glutathione. Microchim Acta 189, 457 (2022). https://doi.org/10.1007/s00604-022-05576-z

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