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Highly selective colorimetric determination of glutathione based on sandwich-structured nanoenzymes composed of gold nanoparticle–coated molecular imprinted metal–organic frameworks

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Abstract

A sandwich-structured composite nanoenzyme (NH2-MIL-101(Fe)@Au@MIP) was prepared using molecularly imprinted polymers, metal–organic frameworks, and gold nanoparticles and a highly selective glutathione (GSH) colorimetric sensor was constructed. The inner part of the composite nanoenzymes is a metal–organic framework loaded with gold nanoparticles (AuNPs), NH2-MIL-101(Fe)@Au, which has superior peroxidase-like activity compared with  NH2-MIL-101(Fe). This is due to the surface plasmon resonance effect of AuNPs. GSH can form strong Au–S bonds with AuNPs, which can significantly reduce the enzymatic activity of NH2-MIL-101(Fe)@Au, thereby changing the absorbance at 450 nm of the sensing system. The degree of change in absorbance is correlated with the concentration of GSH. In the outer part, the molecularly imprinted polymer with oxidized glutathione (GSSG) as a dummy template provided specific pores, which significantly improved the selectivity of the sensing system. The sensor showed good GSH sensing performance in the range 1 ~ 50 μM with a lower limit of detection (LOD) of 0.231 μM and good sensing performance in fetal bovine serum, indicating its high potential for clinical diagnostic applications.

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Data availability

Data cannot be shared for ethical/privacy reasons. The data underlying this article cannot be shared publicly due to the privacy of individuals that participated in the study. The data will be shared on reasonable request to the corresponding author. Correspondence: Tingli Qu E-mail:qutingli@126.com.

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Funding

This work was supported by the Basic Research Program of Shanxi Province (NO. 20210302124586) and Shanxi Provincial Technology Innovation Center Project (NO. 202104010911006).

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Authors and Affiliations

  1. School of Pharmacy, Shanxi Medical University, Taiyuan, 030001, China

    Xiaolin Lu, Liqiu Yan, Xiaoxue Zhou & Tingli Qu

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  1. Xiaolin Lu
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  2. Liqiu Yan
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  3. Xiaoxue Zhou
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  4. Tingli Qu
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Contributions

XL: conceptualization, resources, visualization, data curation, formal analysis, investigation, methodology, writing—original draft; LY: methodology, validation; XZ: investigation; TQ: conceptualization, methodology, funding acquisition, supervision, writing—review and editing.

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Correspondence to Tingli Qu.

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Lu, X., Yan, L., Zhou, X. et al. Highly selective colorimetric determination of glutathione based on sandwich-structured nanoenzymes composed of gold nanoparticle–coated molecular imprinted metal–organic frameworks. Microchim Acta 191, 140 (2024). https://doi.org/10.1007/s00604-023-06167-2

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  • DOI: https://doi.org/10.1007/s00604-023-06167-2

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