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Colorimetric biosensor for visual determination of Golgi protein 73 based on reduced graphene oxide-carboxymethyl chitosan-Hemin/platinum@palladium nanozyme with peroxidase-like activity

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Abstract 

A Golgi protein 73 (GP73) colorimetric biosensor based on the reduced graphene oxide-carboxymethyl chitosan-hemin/platinum@palladium nanoparticles (RGO-CMCS-Hemin/Pt@Pd NPs) with peroxidase-like activity was constructed. The RGO-CMCS-Hemin/Pt@Pd NPs with high peroxidase-like activity were successfully synthesized under mild conditions. Then, the aminylated GP73 aptamer (Apt) was bound to the RGO-CMCS-Hemin/Pt@Pd NPs to form the recognition probe. Another unmodified GP73 aptamer (AptI) was served as the capture probe. In the presence of target GP73, the capture probe and the recognition probe specifically bind to GP73 and form a RGO-CMCS-Hemin/Pt@Pd NP-Apt/GP73/AptI sandwich-type structure, which can oxidase the colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into blue oxTMB in the presence of H2O2. GP73 detection was achieved by measuring the peak UV absorption at 652 nm. Under the optimum conditions, the GP73 concentration was linearly related to the absorbance intensity in the range 10.0–110.0 ng/mL, and the limit of detection (LOD) was 4.7 ng/mL. The proposed colorimetric biosensor was successfully applied to detect GP73 in spiked human serum samples with recoveries of 98.2–107.0% and RSDs of 1.90–5.44%, demonstrating the excellent potential for highly sensitive GP73 detection in clinical detection.

Graphical abstract

A colorimetric biosensor for visual determination of GP73 based on RGO-CMCS-Hemin/Pt@Pd NPs nanozyme with peroxidase-like activity was designed. The GP73 biosensor responses linearly from 10.0–110.0 ng/mL with LOD of 4.7 ng/mL, and shows acceptable specificity and good recovery.

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Funding

This work was supported by the National Nature Science Foundation of China (No. 62161009), Projects of Talents Recruitment of GDUPT (XJ2022000401), the Open Fund of Guangxi Key Laboratory of Information Materials (No. 211022-K), and the Fund of Guangxi Key laboratory of Metabolic Diseases Research (No. 20–065-76).

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Authors and Affiliations

  1. School of Life and Environmental Sciences, Guangxi Key Laboratory of Information Materials, Guilin University of Electronic Technology, Guilin, Guangxi, 541004, People’s Republic of China

    Xinhao Li, Shengnan Li, Qiuyan Lv, Jintao Liang, Zhide Zhou & Guiyin Li

  2. College of Chemistry, Guangdong University of Petrochemical Technology, Guandu Road, Maoming, Guangdong, 525000, People’s Republic of China

    Chaoxian Wang & Guiyin Li

Authors
  1. Xinhao Li
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Contributions

Xinhao LI: Methodology, data curation, writing—original draft preparation. Shengnan LI: Software; writing, original draft preparation; validation. Qiuyan Lv: Methodology, conceptualization. Chaoxian WANG: Visualization, software, data curation. Jintao Liang: Data curation, writing—reviewing and editing. Zhide ZHOU: Supervision, writing—reviewing and editing. Guiyin LI: Supervision, writing—reviewing and editing.

Corresponding authors

Correspondence to Jintao Liang, Zhide Zhou or Guiyin Li.

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The human serum samples used in this study were approved by the Guangxi Key Laboratory of Metabolic Diseases Research Ethics Committee in Guilin, China.

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Highlights

• The RGO-CMCS-Hemin/Pt@Pd NPs with admirable peroxidase-like activity were synthesized.

• GP73 colorimetric sensor based on RGO-CMCS-Hemin/Pt@Pd NPs and aptamer was constructed.

• GP73 detection is achieved by measuring the absorbance changes for TMB-H2O2 system.

• GP73 biosensor responses linearly from 10.0 to 110.0 ng/mL with LOD of 4.7 ng/mL.

• GP73 colorimetric biosensor shows acceptable specificity and good recovery.

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Li, X., Li, S., Lv, Q. et al. Colorimetric biosensor for visual determination of Golgi protein 73 based on reduced graphene oxide-carboxymethyl chitosan-Hemin/platinum@palladium nanozyme with peroxidase-like activity. Microchim Acta 189, 392 (2022). https://doi.org/10.1007/s00604-022-05480-6

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