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Amperometric low potential aptasensor for the fucosylated Golgi protein 73, a marker for hepatocellular carcinoma

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Abstract

The fucosylated Golgi protein 73 (fuc-GP73) has been used as a criterion to distinguish hepatocellular carcinoma (HCC) from other chronic liver diseases. We describe an amperometric aptasensor for ultrasensitive detection of fuc-GP73 that uses a thiolated aptamer against GP73 as the capture probe, and gold nanoparticles (AuNPs) modified with Avidinlens culinaris agglutinin (A-LCA) as the detection probe. The AuNPs on the surface of a gold electrode provide a large surface for immobilization of A-LCA, so that they can be heavily loaded with biotinylated horse radish peroxidase (B-HRP) via avidin-biotin interactions. This results in enhanced analytical sensitivity. Under optimized conditions and a typical working potential as low as 48 mV (vs. SCE), the dynamic response of the electrode covers the 10 pg·mL−1 to 25 ng·mL−1 fuc-GP73 concentation range, with a 7 pg·mL−1 detection limit (for an S/N ratio of 3). The assay is precise, selective and reproducible. It was applied to the determination of fuc-GP73 in serum.

Schematic of an electrochemical aptasensor for the determination of fucosylated golgi protein 73 (fuc-gp73) based on the avidin-Lens culinaris agglutinin (A-LCA) and biotinylated horse radish peroxidase (B-HRP). It was applied to serum analysis with good sensitivity, selectivity and reproducibility.

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Acknowledgements

This work is supported by the National Natural Science Foundation of China (Grant Nos. 81602737, 81672307), and the Science Foundation of Jiangsu Province, China (Grant No. BM2015023).

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Authors and Affiliations

  1. State Key Laboratory of Pharmaceutical Biotechnology, Department of Biochemistry, Nanjing University, Nanjing, 210093, People’s Republic of China

    Jinlong Li, Bei Wang, Shiyu Gu & Yang Xiang

  2. Department of Laboratory Medicine, The Second Affiliated Hospital of Southeast University, Nanjing, 210003, People’s Republic of China

    Jinlong Li

  3. Department of Oncology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, 210011, People’s Republic of China

    Yucai Yang & Zhaoxia Wang

Authors
  1. Jinlong Li
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  2. Bei Wang
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  3. Shiyu Gu
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  4. Yucai Yang
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  5. Zhaoxia Wang
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  6. Yang Xiang
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Correspondence to Zhaoxia Wang or Yang Xiang.

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Li, J., Wang, B., Gu, S. et al. Amperometric low potential aptasensor for the fucosylated Golgi protein 73, a marker for hepatocellular carcinoma. Microchim Acta 184, 3131–3136 (2017). https://doi.org/10.1007/s00604-017-2334-9

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  • DOI: https://doi.org/10.1007/s00604-017-2334-9

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