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Colorimetric assay of phosphate using a multicopper laccase-like nanozyme

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Abstract

A new nanozyme (Cu-NADH) is reported composed of Cu-coordinated nicotinamide adenine dinucleotide (NADH) exhibiting laccase-like activity. The Cu-NADH nanozyme had higher heat tolerance and catalytic efficiency than natural laccase, and its catalytic activity can be enhanced by high concentration of Cl ions and it is intensely inhibited by phosphate. Therefore, a colorimetric method based on Cu-NADH and indigo carmine was successfully developed to detect phosphate in water. This method showed an excellent selectivity for phosphate, and it had a linear relationship in the phosphate concentration range 2–50 μM with a detection limit of 0.37 μM. We believe that this example of coordination between metal ions and biomolecules to mimic natural enzymes can inspire more effective and alternative strategies in nanozyme design and expand their use in sensing and determination.

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Funding

This work was supported by grants from the National Natural Science Foundation of China [82172100] and the Project of Science and Technology Department of Jilin Province, China [No. 20170204027NY, 20200801015GH, 20200404100YY, 20210402039GH].

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Author notes

  1. Sijun Huang and Xuyong Tang contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, China

    Sijun Huang, Xuyong Tang, Liqiang Yu, Shiyin Hong, Jihuan Liu, Yi Guo & Li Xu

  2. Department of Neurosurgery, First Hospital of Jilin University, Changchun, China

    Baofeng Xu

  3. Department of VIP Unit, China-Japan Union Hospital of Jilin University, Changchun, China

    Rui Liu

Authors
  1. Sijun Huang
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  2. Xuyong Tang
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  8. Yi Guo
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  9. Li Xu
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Contributions

Sijun Huang and Xuyong Tang: the equally contributed authors to this work, either the original and revised work. Liqiang Yu and Shiyin Hong: they mainly help to prepare the Cu-NADH nanozymes, evaluate the laccase-like activity, and do some work on real samples determination. Jihuan Liu: who mainly help to investigate the effect of NaCl on Cu-NADH nanozymes. Baofeng Xu and Rui Liu: who mainly help to discuss and review the paper. Yi Guo and Li Xu: they are corresponding authors of this work provide resources and help to write, review, and edit.

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Correspondence to Yi Guo or Li Xu.

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Huang, S., Tang, X., Yu, L. et al. Colorimetric assay of phosphate using a multicopper laccase-like nanozyme. Microchim Acta 189, 378 (2022). https://doi.org/10.1007/s00604-022-05476-2

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