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Immunochromatographic assays based on three kinds of nanoparticles for the rapid and highly sensitive detection of tylosin and tilmicosin in eggs

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Abstract

Three kinds of immunochromatographic assays (ICAs) are proposed for the highly sensitive and rapid determination  of tylosin (TYL) and tilmicosin (TIM) in eggs based on colloidal gold (CG), latex microsphere (LM), and time-resolved fluorescent microsphere (TRFM). Three types of ICAs could tolerate the egg matrix via simple sample pretreatment and demonstrated high sensitivity for TYL and TIM with cut-off values of 6/6/3 μg/kg and 14/14/6 μg/kg, respectively. Furthermore, in a single-blind parallel study 20 egg samples were analyzed  by the three developed ICAs and confirmed by LC–MS/MS. The  results showed good consistency, and there were no false positive and false negative results in our three ICAs. Consequently, the proposed three ICAs offered rapid, highly sensitive, reliable, and selectable testing platforms for screening veterinary medicine or other small molecule contaminants.

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Acknowledgements

This work was supported financially by the National Key Research and Development Program of China (2017YFC1601205), Natural Science Foundation of China (31701703, 31871883), Guangdong and Guangzhou Planned Program in Science and Technology (2017B020207010, 201803020026, and S2013030013338), and Shanxi Provincial Key Research and Development Program (201803D221028-1).

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Author notes

  1. Zhiwei Liu and Jiayi Chen contributed equally to this work.

Authors and Affiliations

  1. Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, 510642, China

    Zhiwei Liu, Jiayi Chen, Yimeng Pang, Xing Shen, Hongtao Lei & Xiangmei Li

  2. China Animal Health and Epidemiology Center, 369 Nanjing Rd, Si Fang Qu, Qingdao, 266032, China

    Sijun Zhao

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  1. Zhiwei Liu
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Contributions

Zhiwei Liu and Jiayi Chen contributed to conceptualization, methodology, and writing—original draft preparation; Sijun Zhao and Yimeng Pang performed data curation; Xing Shen and Hongtao Lei performed validation and writing—review and editing; Xiangmei Li contributed to conceptualization, supervision, project administration, writing—review and editing, and funding acquisition.

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Correspondence to Xiangmei Li.

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Liu, Z., Chen, J., Zhao, S. et al. Immunochromatographic assays based on three kinds of nanoparticles for the rapid and highly sensitive detection of tylosin and tilmicosin in eggs. Microchim Acta 189, 42 (2022). https://doi.org/10.1007/s00604-021-05151-y

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