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Ratiometric fluorescence determination of chlortetracycline based on the aggregation of copper nanoclusters triggered by aluminum ion

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Abstract

The aggregation-induced emission (AIE) characteristic of copper nanoclusters (CuNC) was for the first time used to construct a ratiometric fluorescence probe (CuNC-Al3+) for detection of chlortetracycline (CTC). Aluminum ion (Al3+) can aggregate free CuNC and make it emit a bright and stable red fluorescence. A slight excess of Al3+ in CuNC-Al3+ solution can form a CTC-Al3+ complex to limit the conformational rotation of CTC molecule and enhance CTC fluorescence. So, the red fluorescence of CuNC-Al3+ probe and the enhanced CTC fluorescence are used as a reference signal and a response signal to detect CTC, respectively. The method developed shows a good linear relationship between the CTC concentration and the fluorescence intensity ratio (I495/I575) in the range 0.1-3.0 µM, and the detection limit is 25.3 nM (S/N = 3). In addition, the fluorescent color of CuNC-Al3+ probe changes from red to yellow-green as the concentration of CTC increases. Based on this observation, a fluorescent test paper has also been fabricated.

Graphical abstract

Schematic illustration of Al3+ inducing CuNC to produce AIE performance and detecting CTC.

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Funding

This work was supported by the Guangdong Basic and Applied Basic Research Foundation (No. 2019A1515012169, No. 2021A1515011513) and the National Natural Science Foundation of China (No. 81573678).

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  1. School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, China

    Xin Cai, Jianpin Ye, Ding Duan & Kang Li

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  1. Xin Cai
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Correspondence to Kang Li.

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Cai, X., Ye, J., Duan, D. et al. Ratiometric fluorescence determination of chlortetracycline based on the aggregation of copper nanoclusters triggered by aluminum ion. Microchim Acta 189, 28 (2022). https://doi.org/10.1007/s00604-021-05093-5

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