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A gold/Fe3O4 nanocomposite for use in a surface plasmon resonance immunosensor for carbendazim

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Abstract

A surface plasmon resonance (SPR) biosensor for the pesticide carbendazim is described that has enhanced performance due to the use of a Au/Fe3O4 nanocomposite as an amplifying label on the surface the carboxymethyldextran-coated gold layer of the sensor. The surface was further modified with monoclonal antibody to obtain a sensor for real-time detection of carbendazim. Binding of carbendazim results in a change in refractive index. SPR detection in the absence of Au/Fe3O4 nanocomposite and by UPLC-MS analysis demonstrated the improved performance to be due to the use of the Au/Fe3O4 nanocomposite. Response is linear in the 0.05 to 150 ng·mL−1 carbendazim concentration range, and the limit of detection is 0.44 ng·mL−1. This is more than 1 order of magnitude lower than that of the conventional SPR assay. The recoveries from spiked medlar are between 102.4 and 115.0%. The selectivity was tested by using the pesticides benzimidazole, 2-mercaptobenzimidazole, 2-benzimidazole propionic acid, and 2-(2-aminoethyl) benzimidazole as potential interferents. Conceivably, this Au/Fe3O4 nanocomposite-based method has a large potential for the detection of other small analytes at trace concentrations.

Schematic presentation of the SPR sensor for the detection of the fungicide carbendazim (methyl 2-benzimidazole carbamate; MBC) based on the use of a gold/Fe3O4 nanocomposites.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 81703699, 81573595, and 81603398), CAMS Innovation Fund for Medical Sciences (No. 2016-I2M-1-012 and 2017-I2M-1-013), the Graduate Student Innovation Fund of Peking Union Medical College (No. 2017-1007-14), and National Science and Technology Major Project for ‘Significant New Drugs Development’ (2018ZX09721004-010). The authors acknowledge Beijing Inter-bio Tech CO., LTD for providing the surface plasmon resonance device.

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Authors and Affiliations

  1. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haidian District, Beijing, 100193, China

    Qian Li, Xiaowen Dou, Lei Zhang, Jiaoyang Luo, Xiaoyan Xing & Meihua Yang

  2. Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou, 570311, China

    Xiangsheng Zhao

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  1. Qian Li
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  2. Xiaowen Dou
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  3. Xiangsheng Zhao
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  4. Lei Zhang
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  5. Jiaoyang Luo
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  6. Xiaoyan Xing
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  7. Meihua Yang
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Correspondence to Meihua Yang.

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Li, Q., Dou, X., Zhao, X. et al. A gold/Fe3O4 nanocomposite for use in a surface plasmon resonance immunosensor for carbendazim. Microchim Acta 186, 313 (2019). https://doi.org/10.1007/s00604-019-3402-0

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