Abstract
Broad-spectrum antibodies can effectively recognize substances with similar structures and have broad application prospects in field rapid detection. In this study, broad-spectrum antibodies (Abs) against organophosphorus pesticides (OPs) were used as sensitive recognition elements, which could effectively recognize most OPs. Gold nanoparticles (AuNPs) have good biocompatibility. It combined with Abs to form a gold-labeled probe (AuNPs–Abs), which enhances the effective binding of antibodies to nanomaterials. Prussian blue (PB) was added to electrodeposition solution to enhance the conductivity, resulting in superior electrochemical performance. The AuNP–Abs–PB composite film was prepared by electrodeposition on the electrode surface to improve the anti-interference ability and stability of the immunosensor. Under the optimal experimental conditions, the immunosensor had a wide detection range (IC20–IC80: 1.82 × 10–3–3.29 × 104 ng/mL) and high sensitivity. Most importantly, it was simple to be prepared and could be used to detect multiple OPs.
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References
Chen Z, Xu Y, Li N, Qian Y, Wang Z, Liu Y (2019) A national-scale cumulative exposure assessment of organophosphorus pesticides through dietary vegetable consumption in China. Food Control 104:34–41
Li Y-F, Sun Y-M, Beier RC, Lei H-T, Gee S, Hammock BD, Wang H, Wang Z, Sun X, Shen Y-D, Yang J-Y, Xu Z-L (2017) Immunochemical techniques for multianalyte analysis of chemical residues in food and the environment: a review. TrAC Trends Anal Chem 88:25–40
Zhao F, Yao Y, Jiang C, Shao Y, Barcelo D, Ying Y, Ping J (2020) Self-reduction bimetallic nanoparticles on ultrathin MXene nanosheets as functional platform for pesticide sensing. J Hazard Mater 384:121358
Zhao F, He J, Li X, Bai Y, Ying Y, Ping J (2020) Smart plant-wearable biosensor for in-situ pesticide analysis. Biosens Bioelectron 170:112636
Zhao F, Yao Y, Li X, Lan L, Jiang C, Ping J (2018) Metallic transition metal dichalcogenide nanosheets as an effective and biocompatible transducer for electrochemical detection of pesticide. Anal Chem 90:11658–11664
Liu M, Khan A, Wang ZF, Liu Y, Yang GJ, Deng Y, He NY (2019) Aptasensors for pesticide detection. Biosens Bioelectron 130:174–184
Pundir CS, Malik A, Preety, (2019) Bio-sensing of organophosphorus pesticides: a review. Biosens Bioelectron 140:111348
Jiang X, Li D, Xu X, Ying Y, Li Y, Ye Z, Wang J (2008) Immunosensors for detection of pesticide residues. Biosens Bioelectron 23:1577–1587
Raza N, Kim K-H (2018) Quantification techniques for important environmental contaminants in milk and dairy products. TrAC Trends Anal Chem 98:79–94
Talan A, Mishra A, Eremin SA, Narang J, Kumar A, Gandhi S (2018) Ultrasensitive electrochemical immuno-sensing platform based on gold nanoparticles triggering chlorpyrifos detection in fruits and vegetables. Biosens Bioelectron 105:14–21
Gopinath SC, Tang TH, Citartan M, Chen Y, Lakshmipriya T (2014) Current aspects in immunosensors. Biosens Bioelectron 57:292–302
Liu G, Guo W, Song D (2014) A multianalyte electrochemical immunosensor based on patterned carbon nanotubes modified substrates for detection of pesticides. Biosens Bioelectron 52:360–366
Sanchis A, Salvador JP, Marco MP (2018) Multiplexed immunochemical techniques for the detection of pollutants in aquatic environments. TrAC Trends Anal Chem 106:1–10
Lan M, Guo Y, Zhao Y, Liu Y, Gui W, Zhu G (2016) Multi-residue detection of pesticides using a sensitive immunochip assay based on nanogold enhancement. Anal Chim Acta 938:146–155
Shu Q, Wang L, Ouyang H, Wang W, Liu F, Fu Z (2017) Multiplexed immunochromatographic test strip for time-resolved chemiluminescent detection of pesticide residues using a bifunctional antibody. Biosens Bioelectron 87:908–914
Arduini F, Cinti S, Caratelli V, Amendola L, Palleschi G, Moscone D (2019) Origami multiple paper-based electrochemical biosensors for pesticide detection. Biosens Bioelectron 126:346–354
Zhang J, Fang X, Wu J, Hu Z, Jiang Y, Qi H, Zheng L, Xuan X (2020) An interdigitated microelectrode based aptasensor for real-time and ultratrace detection of four organophosphorus pesticides. Biosens Bioelectron 150:111879
Xu ZL, Wang H, Shen YD, Nichkova M, Lei HT, Beier RC, Zheng WX, Yang JY, She ZG, Sun YM (2011) Conformational changes of hapten-protein conjugates resulting in improved broad-specificity and sensitivity of an ELISA for organophosphorus pesticides. Analyst 136:2512–2520
Khanmohammadi A, Aghaie A, Vahedi E, Qazvini A, Ghanei M, Afkhami A, Hajian A, Bagheri H (2020) Electrochemical biosensors for the detection of lung cancer biomarkers: a review. Talanta 206:120251
Zhou Q, Tang D (2020) Recent advances in photoelectrochemical biosensors for analysis of mycotoxins in food. TrAC Trends Anal Chem 124:115814
Haji-Hashemi H, Safarnejad MR, Norouzi P, Ebrahimi M, Shahmirzaie M, Ganjali MR (2019) Simple and effective label free electrochemical immunosensor for Fig mosaic virus detection. Anal Biochem 566:102–106
Sun C, Liao X, Huang P, Shan G, Ma X, Fu L, Zhou L, Kong W (2020) A self-assembled electrochemical immunosensor for ultra-sensitive detection of ochratoxin A in medicinal and edible malt. Food Chem 315:126289
Ma E, Wang P, Yang Q, Yu H, Pei F, Li Y, Liu Q, Dong Y (2019) Electrochemical immunosensor based on MoS2 NFs/Au@AgPt YNCs as signal amplification label for sensitive detection of CEA. Biosens Bioelectron 142:111580
Viet NX, Hoan NX, Takamura Y (2019) Development of highly sensitive electrochemical immunosensor based on single-walled carbon nanotube modified screen-printed carbon electrode. Mater Chem Phys 227:123–129
Li K, Li X, Fan Y, Yang C, Lv X (2019) Simultaneous detection of gastric cancer screening biomarkers plasma pepsinogen I/II using fluorescent immunochromatographic strip coupled with a miniature analytical device. Sens Actuators B Chem 286:272–281
Huang L, Chen J, Yu Z, Tang D (2020) Self-powered temperature sensor with seebeck effect transduction for photothermal-thermoelectric coupled immunoassay. Anal Chem 92:2809–2814
Pei X, Zhang B, Tang J, Liu B, Lai W, Tang D (2013) Sandwich-type immunosensors and immunoassays exploiting nanostructure labels: a review. Anal Chim Acta 758:1–18
Zhang K, Lv S, Lin Z, Tang D (2017) CdS: Mn quantum dot-functionalized g-C3N4 nanohybrids as signal-generation tags for photoelectrochemical immunoassay of prostate specific antigen coupling DNAzyme concatamer with enzymatic biocatalytic precipitation. Biosens Bioelectron 95:34–40
Zhang K, Lv S, Lin Z, Li M, Tang D (2018) Bio-bar-code-based photoelectrochemical immunoassay for sensitive detection of prostate-specific antigen using rolling circle amplification and enzymatic biocatalytic precipitation. Biosens Bioelectron 101:159–166
Zhang B, Liu B, Tang D, Niessner R, Chen G, Knopp D (2012) DNA-based hybridization chain reaction for amplified bioelectronic signal and ultrasensitive detection of proteins. Anal Chem 84:5392–5399
Yu Z, Tang Y, Cai G, Ren R, Tang D (2019) Paper electrode-based flexible pressure sensor for point-of-care immunoassay with digital multimeter. Anal Chem 91:1222–1226
Puertas S, de Gracia VM, Mendoza E, Jimenez-Jorquera C, de la Fuente JM, Fernandez-Sanchez C, Grazu V (2013) Improving immunosensor performance through oriented immobilization of antibodies on carbon nanotube composite surfaces. Biosens Bioelectron 43:274–280
Lah Z, Ahmad SAA, Zaini MS, Kamarudin MA (2019) An electrochemical sandwich immunosensor for the detection of HER2 using antibody-conjugated PbS quantum dot as a label. J Pharm Biomed Anal 174:608–617
Gao Z, Xu M, Hou L, Chen G, Tang D (2013) Magnetic bead-based reverse colorimetric immunoassay strategy for sensing biomolecules. Anal Chem 85:6945–6952
Jampasa S, Lae-Ngee P, Patarakul K, Ngamrojanavanich N, Chailapakul O, Rodthongkum N (2019) Electrochemical immunosensor based on gold-labeled monoclonal anti-LipL32 for leptospirosis diagnosis. Biosens Bioelectron 142:111539
Chen H, Yang T, Liu F, Li W (2019) Electrodeposition of gold nanoparticles on Cu-based metal-organic framework for the electrochemical detection of nitrite. Sens Actuators B Chem 286:401–407
Dou X, Chu X, Kong W, Luo J, Yang M (2015) A gold-based nanobeacon probe for fluorescence sensing of organophosphorus pesticides. Anal Chim Acta 891:291–297
Makaraviciute A, Ramanaviciene A (2013) Site-directed antibody immobilization techniques for immunosensors. Biosens Bioelectron 50:460–471
Perez-Fernandez B, Mercader JV, Abad-Fuentes A, Checa-Orrego BI, Costa-Garcia A, Escosura-Muniz A (2020) Direct competitive immunosensor for Imidacloprid pesticide detection on gold nanoparticle-modified electrodes. Talanta 209:120465
Samadi Pakchin P, Fathi M, Ghanbari H, Saber R, Omidi Y (2020) A novel electrochemical immunosensor for ultrasensitive detection of CA125 in ovarian cancer. Biosens Bioelectron 153:112029
Husmann S, Orth ES, Zarbin AJG (2019) A multi-technique approach towards the mechanistic investigation of the electrodeposition of Prussian blue over carbon nanotubes film. Electrochim Acta 312:380–391
Haji-Hashemi H, Habibi MM, Safarnejad MR, Norouzi P, Ganjali MR (2018) Label-free electrochemical immunosensor based on electrodeposited Prussian blue and gold nanoparticles for sensitive detection of citrus bacterial canker disease. Sens Actuators B Chem 275:61–68
Fu J, An X, Yao Y, Guo Y, Sun X (2019) Electrochemical aptasensor based on one step co-electrodeposition of aptamer and GO-CuNPs nanocomposite for organophosphorus pesticide detection. Sens Actuators B Chem 287:503–509
Ping J, Mao X, Fan K, Li D, Ru S, Wu J, Ying Y (2010) A Prussian blue-based amperometric sensor for the determination of hydrogen peroxide residues in milk. Ionics 16:523–527
Ping J, Wu J, Fan K, Ying Y (2011) An amperometric sensor based on Prussian blue and poly(o-phenylenediamine) modified glassy carbon electrode for the determination of hydrogen peroxide in beverages. Food Chem 126:2005–2009
Shaikh MO, Zhu PY, Wang CC, Du YC, Chuang CH (2019) Electrochemical immunosensor utilizing electrodeposited Au nanocrystals and dielectrophoretically trapped PS/Ag/ab-HSA nanoprobes for detection of microalbuminuria at point of care. Biosens Bioelectron 126:572–580
Yao Y, Wang G, Chu G, An X, Guo Y, Sun X (2019) The development of a novel biosensor based on gold nanocages/graphene oxide–chitosan modified acetylcholinesterase for organophosphorus pesticide detection. New J Chem 43:13816–13826
Tian X, Liu L, Li Y, Yang C, Zhou Z, Nie Y, Wang Y (2018) Nonenzymatic electrochemical sensor based on CuO–TiO2 for sensitive and selective detection of methyl parathion pesticide in ground water. Sens Actuators B Chem 256:135–142
Mahmoudi E, Fakhri H, Hajian A, Afkhami A, Bagheri H (2019) High-performance electrochemical enzyme sensor for organophosphate pesticide detection using modified metal-organic framework sensing platforms. Bioelectrochemistry 130:107348
Acknowledgements
This work was supported by the National Natural Science Foundation of China (31772068), Special Project of Independent Innovation of Shandong Province (2018CXGC0214), Shandong Provincial Natural Science Foundation (ZR2018ZC0126).
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Dong, H., Zhao, Q., Li, J. et al. Broad-spectrum electrochemical immunosensor based on one-step electrodeposition of AuNP–Abs and Prussian blue nanocomposite for organophosphorus pesticide detection. Bioprocess Biosyst Eng 44, 585–594 (2021). https://doi.org/10.1007/s00449-020-02472-9
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DOI: https://doi.org/10.1007/s00449-020-02472-9