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Electrochemical immunosensing using a nanostructured functional platform for determination of α-zearalanol

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Abstract

We describe an electrochemical immunosensor for the determination of the growth promoter α-zearalanol in bovine serum. The sensing scheme is based on a nanocomposite consisting of gold nanoparticles electrodeposited on multi-walled carbon nanotubes that were modified with poly (vinylpyridine) through in-situ polymerization. The electrodeposition of the gold nanoparticles enlarges the surface available for immobilization of antibodies against α-zearalanol. The nanocomposite film was characterized by scanning electron microscopy, energy dispersive X-ray spectroscopy, and cyclic voltammetry. The calibration plot has a linear response in the concentrations range from 0.05 to 50 ng mL−1, and the detection limit is 16 pg mL−1. The time required for analysis is 12 min only which compares quite favorably with the time (90 min) required by the conventional ELISA. The method exhibits good selectivity, stability and reproducibility for detecting α-zearalanol in the livestock production.

Schematic representation of the immunocapture procedure and subsequent determination of α-zearalanol.

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Acknowledgments

The authors wish to thank the financial support from Universidad Nacional de San Luis (UNSL), Instituto de Química de San Luis (INQUISAL), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA).

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Authors and Affiliations

  1. INQUISAL-CONICET, Departamento de Química, Universidad Nacional de San Luis, Chacabuco, 917, D5700BWS, San Luis, Argentina

    Matías Regiart, Marco A. Seia, Germán A. Messina, Franco A. Bertolino & Julio Raba

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  1. Matías Regiart
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  2. Marco A. Seia
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  3. Germán A. Messina
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  4. Franco A. Bertolino
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  5. Julio Raba
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Corresponding authors

Correspondence to Franco A. Bertolino or Julio Raba.

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Regiart, M., Seia, M.A., Messina, G.A. et al. Electrochemical immunosensing using a nanostructured functional platform for determination of α-zearalanol. Microchim Acta 182, 531–538 (2015). https://doi.org/10.1007/s00604-014-1355-x

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  • DOI: https://doi.org/10.1007/s00604-014-1355-x

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