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A terbium-based metal-organic framework@gold nanoparticle system as a fluorometric probe for aptamer based determination of adenosine triphosphate

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Abstract

This study reports on a method for fluorometric aptasensing of adenosine triphosphate (ATP). It is based on the interaction of dispersed (red) and agglomerated (blue) gold nanoparticles (AuNPs) with a water-dispered terbium(III) based metal-organic framework (Tb-MOF). The dispersed AuNPs quench the emissions of the Tb-MOF, while the aggregated AuNPs have little effect. Under the condition of high salt concentration, the free aptamer against ATP does not stabilize the AuNPs against aggregation. This causes a color change from red to blue and weak quenching of the fluorescence of the Tb-MOF (with peaks at 489 nm and 544 nm after excitation at 290 nm). On addition of ATP, it will be bound by its aptamer to form a complex that is adsorbed on the AuNPs. This protects the AuNPs from salt-induced aggregation and the color (with a peak at 525 nm) remains red. The two fluorescence bands of the Tb-MOF are therefore suppressed by fluorescence resonance energy transfer (FRET) between Tb-MOF and the dispersed AuNPs. Fluorescence drops linearly in the 50 nM to 10 μM ATP concentration range, and the detection limit is 23 nM. ATP analogs such as guanosine triphosphate, uridine triphosphate, cytidine triphosphate, adenosine monophosphate and cyclic adenosine monophosphate have no obvious interference. The method was successfully applied to the determination of ATP in (spiked) human plasma samples and gave satisfactory recoveries.

Schematic of a terbium-based metal-organic framework@gold nanoparticle system as a fluorometric probe for aptamer based determination of adenosine triphosphate. The dispersed gold nanoparticles (AuNPs) quench the fluorescence of the terbium-based metal-organic framework (Tb-MOF), while the aggregated AuNPs have little effect. In the presence of adenosine triphosphate (ATP), the aptamer-ATP complexes provide greater protection towards AuNPs than aptamer alone under high salt condition. Based on this, a novel Tb-MOF@AuNP platform is established for ATP detection.

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References

  1. Wang LD, Wang SX, Chen Y (2017) Detection of cyanide via extended Π-conjugation-induced fluorescence enhancement of a metal organic framework composed of terbium(III), bipyridyl and adenosine diphosphate. Microchim Acta 184:4597–4602

    Article  CAS  Google Scholar 

  2. Zhang F, Zhang GW, Yao H, Wang Y, Chu TS, Yang YY (2017) A europium (III) based nano-flake MOF film for efficient fluorescent sensing of picric acid. Microchim Acta 184:1207–1213

    Article  CAS  Google Scholar 

  3. Dang S, Wang T, Yi FY, Liu QH, Yang WT, Sun ZM (2015) A nanoscale multiresponsive luminescent sensor based on a terbium(III) Metal-organic Framework. Chem Asin J 10(8):1703–1709

    Article  CAS  Google Scholar 

  4. Pal S, Bharadwaj PK (2016) A luminescent terbium MOF containing hydroxyl groups exhibits selective sensing of nitroaromatic compounds and Fe(III) ions. Cryst Growth Des 16(10):5852–5858

    Article  CAS  Google Scholar 

  5. Yang ZR, Wang MM, Wang XS, Yin XB (2017) Boric-acid-functional lanthanide metal-organic frameworks for selective ratiometric fluorescence detection of fluoride ions. Anal Chem 89(3):1930–1936

    Article  CAS  PubMed  Google Scholar 

  6. Qian JJ, Qiu LG, Wang YM, Yuan YP, Xie AJ, Shen YH (2014) Fabrication of magnetically separable fluorescent terbium-based MOF nanospheres for highly selective trace-level detection of TNT. Dalton Transactions 43(10):3978–3983

    Article  CAS  PubMed  Google Scholar 

  7. Tedsana W, Tuntulani T, Ngeontae W (2013) A highly selective turn-on ATP fluorescence sensor based on unmodified cysteamine capped CdS quantum dots. Anal Chim Acta 783:65–73

    Article  CAS  PubMed  Google Scholar 

  8. Zheng FF, Zhang PH, Yu X, Chen JJ, Li LL, Zhu JJ (2015) Aptamer/graphene quantum dots nanocomposite capped fluorescent mesoporous silica nanoparticles for intracellular drug delivery and real-time monitoring of drug release. Anal Chem 87(23):11739–11745

    Article  CAS  PubMed  Google Scholar 

  9. Lu LM, Zhang XB, Kong RM, Yang B, Tan WH (2011) A ligation-triggered DNAzyme cascade for amplified fluorescence detection of biological small molecules with zero-background signal. J Am Chem Soc 133(30):11686–11169

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Zhang ZX, Balogh D, Wang F, Willner I (2013) Smart mesoporous SiO2 nanoparticles for the DNAzyme-induced multiplexed release of substrates. J Am Chem Soc 135(5):1934–1940

    Article  CAS  PubMed  Google Scholar 

  11. Berg J, Hung YP, Yellen G (2009) A genetically encoded fluorescent reporter of ATP: ADP ratio. Nat Methods 6(2):161–166

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Muthuraja B, Chowdhurya SR, Mukherjeebc S, Patra CR, Iyer PK (2015) Aggregation deaggregation influenced selective and sensitive detection of Cu2+ and ATP by histidine functionalized water-soluble fluorescent perylene diimide under physiological conditions and in living cells. RSC Adv 5(36):28211–28218

    Article  CAS  Google Scholar 

  13. He XX, Zhao YX, He DG, Wang KM, Xu FZ, Tang JL (2012) ATP-responsive controlled release system using aptamer-functionalized mesoporous silica nanoparticles. Langmuir 28(35):12909–12915

    Article  CAS  PubMed  Google Scholar 

  14. Li F, Du ZF, Yang LM, Tang B (2013) Selective and sensitive turn-on detection of adenosine triphosphate and thrombin based on bifunctional fluorescent oligonucleotide probe. Biosens Bioelectron 41:907–910

    Article  CAS  PubMed  Google Scholar 

  15. Gao PY, Xia YF, Yang LL, Ma TF, Yang L, Guo QQ, Huang SS (2014) Aptasensor for adenosine triphosphate based on electrode-supported lipid bilayer membrane. Microchim Acta 181(1–2):205–212

    Article  CAS  Google Scholar 

  16. Huo Y, Qi L, Lv XJ, Lai T, Zhang J, Zhang ZQ (2016) A sensitive aptasensor for colorimetric detection of adenosine triphosphate based on the protective effect of ATP-aptamer complexes on unmodified gold nanoparticles. Biosens Bioelectron 78:315–320

    Article  CAS  PubMed  Google Scholar 

  17. Kimling J, Maier M, Okenve B, Kotaidis V, Ballot H, Plech A (2006) Turkevich method for gold nanoparticle synthesis revisited. J Phys Chem B 110:15700–15707

    Article  CAS  PubMed  Google Scholar 

  18. Haiss W, Thanh NTK, Aveyard J, and G. Fernig D (2007) Determination of size and concentration of gold nanoparticles from UV-Vis spectra. Anal Chem 79(11): 4215-4221

  19. Abdelhamid HN, Bermejo-Gómez A, Martín-Matute B, Zou XD (2017) A water-stable lanthanide metal-organic framework for fluorimetric detection of ferric ions and tryptophan. Microchim Acta 184:3363–3371

    Article  CAS  Google Scholar 

  20. Yu XS, Hu LZ, Zhang F, Wang M, Xia ZN, Wei WL (2018) MoS2 quantum dots modified with a labeled molecular beacon as a ratiometric fluorescent gene probe for FRET based detection and imaging of microRNA. Microchim Acta 185:239

    Article  CAS  Google Scholar 

  21. He JC, Li GK, Hu YL (2017) Aptamer-involved fluorescence amplification strategy facilitated by directional enzymatic hydrolysis for bioassays based on a metal-organic framework platform: highly selective and sensitive determination of thrombin and oxytetracycline. Microchim Acta 184:2365–2373

    Article  CAS  Google Scholar 

  22. Takakusa H, Kikuchi K, Urano Y, Kojima H, Nagano T (2003) A novel design method of ratiometric fluorescent probes based on fluorescence resonance energy transfer switching by spectral overlap integral. Chem Eur J 9(7):1479–1485

    Article  CAS  PubMed  Google Scholar 

  23. Dhasa TS, Kumara VG, Karthicka V, Govindarajua K, Narayana TS (2014) Biosynthesis of gold nanoparticles using Sargassum swartzii and its cytotoxicity effect on HeLa cells. Spectrochim Acta A 133:102–106

    Article  CAS  Google Scholar 

  24. Jiang GY, Zhu WY, Shen X, Xu L, Li XX, Wang R, Liu CY, Zhou XM (2017) Colorimetric and visual determination of adenosine triphosphate using a boronic acid as the recognition element, and based on the deaggregation of gold nanoparticles. Microchim Acta 184(11):4305–4312

    Article  CAS  Google Scholar 

  25. Liu XJ, Lin BX, Yu Y, Cao YJ, Guo ML (2018) A multifunctional probe based on the use of labeled aptamer and magnetic nanoparticles for fluorometric determination of adenosine 5′-triphosphate. Microchim Acta 185:243

    Article  CAS  Google Scholar 

  26. Zhu Y, Hu XC, Shi S, Gao RR, Huang HL, Zhu YY, Lv XY, Yao TM (2016) Ultrasensitive and universal fluorescent aptasensor for the detection of biomolecules (ATP, adenosine and thrombin) based on DNA/Ag nanoclusters fluorescence light-up system. Biosens Bioelectron 79:205–212

    Article  CAS  PubMed  Google Scholar 

  27. Cheng X, Cen Y, Xu GH, Wei FD, Shi ML, Xu XM, Sohail M, Hu Q (2018) Aptamer based fluorometric determination of ATP by exploiting the FRET between carbon dots and graphene oxide. Microchim Acta 185:144

    Article  CAS  Google Scholar 

  28. Ning Y, Wei K, Cheng LJ, Hu J, Xiang Q (2017) Fluorometric aptamer based determination of adenosine triphosphate based on deoxyribonuclease I-aided target recycling and signal amplification using graphene oxide as a quencher. Microchim Acta 184(6):1847–1854

    Article  CAS  Google Scholar 

  29. Qiu HZ, Liu ZE, Huang ZJ, Chen M, Cai XH, Weng SH, Lin XH (2015) Aptamer based turn-off fluorescent ATP assay using DNA concatamers. Microchim Acta 182:2387–2393

    Article  CAS  Google Scholar 

  30. Li X, Peng Y, Chai YQ, Yuan R, Xiang Y (2016) A target responsive aptamer machine for label-free and sensitive non-enzymatic recycling amplification detection of ATP. Chem Commun 52:3673–3676

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Youth Fund Project) (21405093), Higher Educational Science and Technology Program of Shandong Province, China (Grant No. J15LC04), the Scientific Research Foundation of Qufu Normal University (BSQD20130117), and the Experimental Research Project of Qufu Normal University (jp201716).

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  1. Fei Qu and Chao Sun contributed equally to this work.

Authors and Affiliations

  1. The Key Laboratory of Life-Organic Analysis, Qufu Normal University, Qufu, 273165, Shandong, China

    Fei Qu, Chao Sun, Xiaoxia Lv & Jinmao You

  2. The Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University, Qufu, 273165, Shandong, China

    Fei Qu, Chao Sun, Xiaoxia Lv & Jinmao You

  3. Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, China

    Jinmao You

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  1. Fei Qu
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  2. Chao Sun
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Correspondence to Fei Qu.

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Qu, F., Sun, C., Lv, X. et al. A terbium-based metal-organic framework@gold nanoparticle system as a fluorometric probe for aptamer based determination of adenosine triphosphate. Microchim Acta 185, 359 (2018). https://doi.org/10.1007/s00604-018-2888-1

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