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A multifunctional probe based on the use of labeled aptamer and magnetic nanoparticles for fluorometric determination of adenosine 5’-triphosphate

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Abstract

A multifunctional fluorescent probe is synthesized for the determination of adenosine 5′-triphosphate (ATP). The 6-carboxyfluorescein-labeled aptamer (FAM-aptamer) was bound to the surface of magnetite nanoparticles coated with polydopamine (Fe3O4@PDA) by π-π stacking interaction to form the multifunctional probe. The probe has three functions including recognition, magnetic separation, and yielding a fluorescent signal. In the presence of ATP, FAM-aptamer on the surface of the probe binds to ATP and returns to the solution. Thus, the fluorescence of the supernatant is enhanced and can be related to the concentration of ATP. Fluorescence intensities were measured at excitation/emission wavelengths of 494/526 nm. Response is linear in the 0.1–100 μM ATP concentration range, and the detection limit is 89 nM. The probe was applied to the quantitation of ATP in spiked human urine and serum samples, with recoveries ranging between 94.8 and 102%.

A multifunctional fluorescent probe based on the use of FAM-aptamer and Fe3O4@PDA is described for the determination of ATP in spiked human urine and serum samples. FAM-aptamer: 6-carboxyfluorescein-labeled aptamer; Fe3O4@PDA: magnetite nanoparticles coated with polydopamine. ATP: adenosine 5′-triphosphate.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Nos. 21575043, 21275056, 21605052, 51478196); and the Platform Construction Project of Guangzhou Science Technology and Innovation Commission (No. 15180001).

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  1. Ying Yu

    Present address: , Guangzhou, People’s Republic of China

Authors and Affiliations

  1. Key Laboratory of Analytical Chemistry for Biomedicine,School of Chemistry and Environment, South China Normal University, Guangzhou, Guangdong, 510006, China

    Xiaojie Liu, Bixia Lin, Ying Yu, Yujuan Cao & Manli Guo

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Correspondence to Ying Yu.

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Liu, X., Lin, B., Yu, Y. et al. A multifunctional probe based on the use of labeled aptamer and magnetic nanoparticles for fluorometric determination of adenosine 5’-triphosphate. Microchim Acta 185, 243 (2018). https://doi.org/10.1007/s00604-018-2774-x

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