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A gold electrode modified with a nanoparticulate film composed of a conducting copolymer for ultrasensitive voltammetric sensing of hydrogen peroxide

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Abstract

A film consisting of poly(γ-glutamic acid) modified with 3-aminothiophene (ATh-γ-PGA) was prepared by macromolecular self-assembly and electropolymerization. ATh-γ-PGA is amphiphilic and electrically conductive. The copolymers undergo self-assembly to form nanoparticles (NPs) on decreasing the pH value of an aqueous solution. A conducting film of NPs was formed on the surface of a gold electrode by casting the ATh-γ-PGA NPs and subsequently electropolymerizing the thiophene units. Next, horseradish peroxidase and Nafion were cast onto the film to obtain an enzymatic biosensor for H2O2. Due to the electropolymerization step, a cross-conjugated polymer network is created that improves electron transfer rates and thus enhances the response. This endows the biosensor with high sensitivity. Two linear ranges are present, the first ranging from 1 × 10−11 to 1 × 10−8 mol·L−1, and the second from 1 × 10−8 to 1 × 10−5 mol·L−1. The detection limit is as low as 3 × 10−12 mol·L−1. The sensor is stable, repeatable, and was successfully applied to the determination of H2O2 in a commercial disinfecting solution.

Preparation of a conducting nanoparticle (NP) film on the gold electrode (GE) by self-assembly of poly(γ-glutamic acid) that was modified with electroactive 3-aminothiophene (ATh-γ-PGA). It served as a platform for the fabricationof an ultrasensitive voltammetric enzyme-based biosensor for H2O2.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (NSFC 21504001, 51572004), the foundation of Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, Jiangnan University (JDSJ2015-02), Natural Science Foundation of the Higher Education Institutions of Anhui Province (KJ2016SD06, KJ2017A115) and the Anhui Provincial Natural Science Research Program of High Education Improvement Plan (TSKJ2016B17).

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Authors and Affiliations

  1. School of Biological and Chemical Engineering, Anhui Polytechnic University, Wuhu, 241000, China

    Rongli Zhang, Can Jiang, Renchun Yang, Yanyan Sun & Cuige Zhang

  2. Xi’an North Huitian Chemical Industry Co. Ltd., Xi’an, 710302, China

    Xiaoxia Fan

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  1. Rongli Zhang
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  2. Can Jiang
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  3. Xiaoxia Fan
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  4. Renchun Yang
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Correspondence to Rongli Zhang or Cuige Zhang.

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Zhang, R., Jiang, C., Fan, X. et al. A gold electrode modified with a nanoparticulate film composed of a conducting copolymer for ultrasensitive voltammetric sensing of hydrogen peroxide. Microchim Acta 185, 58 (2018). https://doi.org/10.1007/s00604-017-2564-x

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