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Visual and rapid lateral flow immunochromatographic assay for enrofloxacin using dyed polymer microspheres and quantum dots

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Abstract

The authors describe two kinds of immunochromatographic assays. The first is based on the use of dyed polymer microspheres (MICA), the another on the use of quantum dots (QICA). Both enable visual detection of enrofloxacin (ENR) in animal tissue and milk. Both the MICA and the QICA have visual limits of detection of 1 μg·L−1 when working in buffer, of 5 μg·kg−1 in case of animal tissue, and of 10 μg·L−1 in case of milk. Other quinolones do not interfere. The MICA and QICA described here are convenient and fairly rapid in that the detection process (including sample pretreatment and assay) takes 20 min only which is far less than the commercial enzyme-linked immunosorbent assay (ELISA) test kit which requires 120 min. The MICA and QICA are more sensitive than the immunochromatographic assay using colloidal gold labels and the same polyclonal antibody. The results of analysis of spiked samples via MICA and QICA are in good agreement with those obtained by the commercial ELISA test kit.

A visual dyed polymer microsphere-based immunochromatographic assay (MICA) and a visual quantum-dot-based immunochromatographic assay (QICA) are described for the rapid, specific, qualitative, semi-quantitative, and on-site detection of enrofloxacin residues in animal origin foods.

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Acknowledgements

This work was supported by the National Key Research and Development Program of China (Project No. 2016YFD0401204), the National Natural Science Foundation of China (Project No. 31201353), and Tianjin Municipal Science and Technology Commission (Project No. 13JCQNJC15300 and 16PTSYJC00130).

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Author notes

  1. Wei Sheng and Shijie Li contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin, 300457, China

    Wei Sheng, Shijie Li, Yue Liu, Junping Wang, Yan Zhang & Shuo Wang

  2. Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University (BTBU), Beijing, 100048, China

    Shuo Wang

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  1. Wei Sheng
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  2. Shijie Li
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  3. Yue Liu
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  4. Junping Wang
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  5. Yan Zhang
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  6. Shuo Wang
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Correspondence to Wei Sheng or Shuo Wang.

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Sheng, W., Li, S., Liu, Y. et al. Visual and rapid lateral flow immunochromatographic assay for enrofloxacin using dyed polymer microspheres and quantum dots. Microchim Acta 184, 4313–4321 (2017). https://doi.org/10.1007/s00604-017-2474-y

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  • DOI: https://doi.org/10.1007/s00604-017-2474-y

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