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Aptamer-based impedimetric determination of the human blood clotting factor IX in serum using an interdigitated electrode modified with a ZnO nanolayer

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Abstract

This article describes a sensitive impedimetric method for the determination of human blood coagulation factor IX protein (FIX) which is present in extremely low concentration in serum. An interdigitated electrode (IDE) whose surface was layered with zinc oxide was modified with two kinds of probes. One is an antibody, the other an aptamer against FIX. A comparative study between anti-FIX aptamer and anti-FIX antibody showed the aptamer to possess higher affinity for FIX. A sandwich aptamer assay was worked out by using the FIX-binding aptamer on the surface of the IDE. It has a detection limit as low as 10 pM which makes it 4 to 30-fold more sensitive than any other method reported for FIX. Moreover, to practice detection in clinical samples, FIX was detected from the human blood serum by spiking. In our perception, the sensitivity of the ZnO-modified IDE presented here makes it a promising tool for sensing clinically relevant analytes that are present in very low (sub-pM) concentrations.

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Acknowledgments

Y. Chen thanks the support from High Impact Research grants - “MoE Grant UM.C/625/1/HIR/MOE/DENT/09” and “UM-MoHE HIR UM.C/625/1/HIR/MOHE/MED/16/5”

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Authors and Affiliations

  1. Institute of Nano Electronic Engineering (INEE), Universiti Malaysia Perlis, 01000, Kangar, Perlis, Malaysia

    Ong Chong Cheen, Subash C. B. Gopinath, Veeradasan Perumal, M. K. Md Arshad, Thangavel Lakshmipriya, R. Haarindraprasad, Balakrishnan S. Rao & Uda Hashim

  2. Faculty of Engineering Technology, Universiti Malaysia Perlis, 01000, Kangar, Perlis, Malaysia

    Ong Chong Cheen

  3. School of Bioprocess Engineering, Universiti Malaysia Perlis, 02600, Arau, Perlis, Malaysia

    Subash C. B. Gopinath

  4. Centre of Innovative Nanostructure & Nanodevices (COINN), Universiti Teknologi PETRONAS, 32610, Bandar Seri Iskandar, Perak Darul Ridzuan, Malaysia

    Veeradasan Perumal

  5. Department of Oral Biology & Craniofacial Sciences, Kuala Lumpur, Malaysia

    Yeng Chen

  6. Oral Cancer Research and Coordinating Center (OCRCC), Faculty of Dentistry, University of Malaya, 50603, Kuala Lumpur, Malaysia

    Yeng Chen

  7. Fakulti Kejuruteraan dan Alam Bina, Universiti Sains Islam Malaysia (USIM), Bandar Baru Nilai, 71800, Nilai, Negeri Sembilan, Malaysia

    Balakrishnan S. Rao

  8. Department of Inorganic Chemistry, University of Madras, Guindy Campus, Chennai, 600 025, Tamil Nadu, India

    Kannaiyan Pandian

Authors
  1. Ong Chong Cheen
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  2. Subash C. B. Gopinath
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  3. Veeradasan Perumal
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  4. M. K. Md Arshad
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  5. Thangavel Lakshmipriya
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  6. Yeng Chen
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  7. R. Haarindraprasad
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  8. Balakrishnan S. Rao
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  9. Uda Hashim
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Correspondence to Subash C. B. Gopinath.

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Cheen, O.C., Gopinath, S.C.B., Perumal, V. et al. Aptamer-based impedimetric determination of the human blood clotting factor IX in serum using an interdigitated electrode modified with a ZnO nanolayer. Microchim Acta 184, 117–125 (2017). https://doi.org/10.1007/s00604-016-2001-6

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  • DOI: https://doi.org/10.1007/s00604-016-2001-6

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